文献类型：期刊文献

英文题名：Differences in Toxicity Produced by the Various Polymer Types of Nanoplastics on Hepg2 Cells

作者：Ma, Lihua[1]; Wu, Zijie[1]; Lu, Zifan[2]; Yan, Linhong[1]; Dong, Xiaoling[1]; Dai, Zhenqing[3,4]; Sun, Ruikun[2]; Hong, Pengzhi[1]; Zhou, Chunxia[1]; Li, Chengyong[2,3,4,5]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang, 524088, China; [2] School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, 524088, China; [3] Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518108, China; [4] Guangdong Provincial Key Laboratory of Intelligent Equipment for South China Sea Marine Ranching, Guangdong Ocean University, Zhanjiang, 524088, China; [5] Guangdong Provincial Observation and Research Station for Tropical Ocean Environment in Western Coastal Water, Guangdong Ocean University, Zhanjiang, 524088, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230401646)

语种：英文

外文关键词：Chlorine compounds - DNA - Emulsification - Genes - Health risks - Metabolism - Microplastic - Particle size - Particle size analysis - Plastic bottles - Plastic recycling - Polyethylene terephthalates - Polyethylenes - Polyvinyl chlorides - Repair

外文摘要：The problem of microplastics (MPs) contamination in food has gradually come to the fore. MPs can be transmitted through the food chain and accumulate within various organisms, ultimately posing a threat to human health. For the first time, we compared the differences in toxicity of polyethylene terephthalate (PET), polyvinyl chloride (PVC), and polystyrene (PS) nanoplastics (NPs) of different polymer types to HepG2 cells and their potential toxic mechanisms. Common polymers PET, PVC, and PS NPs, which had similar particle size, surface charge, and shape, were prepared using nanoprecipitation and emulsion polymerization successfully. This enabled us to investigate the impact of different polymer types on cytotoxicity. The findings demonstrated that high concentrations of PET and PVC NPs can trigger cell apoptosis and oxidative stress, but PS NPs did not have the same harmful impact. Furthermore, our analysis of transcriptomics data revealed that various types of NPs significantly reduced the expression of genes related to bile acid metabolism in the PPAR signaling pathway. This effect may lead to the perturbation of cellular lipid metabolism. PET and PVC NPs resulted in a higher degree of down-regulation of these genes compared to PS NPs. It is worth noting that PET and PVC NPs can significantly inhibit the expression of cell cycle and DNA repair-related genes in the p53 signaling pathway, aggravating DNA damage and arresting cell cycle progression by inhibiting DNA repair. This may constitute the principal factor accounting for variations in NPs toxicity across different polymer types. These comparative toxicity studies and discoveries regarding molecular mechanisms provide a theoretical basis for mitigating human health risks stemming from NPs. ? 2023, The Authors. All rights reserved.