文献类型：期刊文献

中文题名：鳗鱼纳米粒子-花色苷复合物对HepG2细胞氧化损伤的保护作用

英文题名：Protective Effect of Eel Nanoparticle-anthocyanin Complex on Oxidative Damage in HepG2 Cells

作者：滕慧[1,3];吴婷[2];张畅[1];陈雷[1,3]

机构：[1]广东海洋大学食品科技学院/广东省水产品加工与安全重点实验室/广东省海洋食品工程技术研究中心/广东省现代农业科技创新中心/广东省海洋生物制品工程实验室,广东湛江524088;[2]福建农林大学食品科学学院,福建福州350002;[3]海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁大连116034

年份：2023

卷号：43

期号：1

起止页码：103

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD、、CSCD_E2023_2024、北大核心、CSCD、北大核心2020

基金：国家自然科学基金面上项目(32072209)。

语种：中文

中文关键词：鳗鱼;纳米粒子;花色苷;氧化应激;保护作用

外文关键词：eel;nanoparticles;anthocyanins;oxidative damage;protective effect

中文摘要：【目的】建立鳗鱼(Muraenesox cinereus)纳米粒子运载体系,探究其对HepG2人肝癌细胞氧化损伤的保护作用。【方法】制备鳗鱼纳米粒子-花色苷复合物(ENPs-C3G)运载体系,并测定其ABTS+清除能力以及FRAP还原力,利用H2O2建立HepG2氧化应激模型,并探究ENPs-C3G对H2O2诱导的HepG2细胞内活性氧(ROS)、NO质量摩尔浓度以及超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力水平的影响。【结果】ENPs-C3G复合物对ABTS+清除能力显著高于CYD-3-G(P<0.05),复合物质量浓度比为1∶20组ENPs-C3G的ABTS+清除能力最强,接近100%;ENPs-C3G对亚铁离子还原力高于CYD-3-G,1∶40组ENPs-C3G亚铁离子还原力为(0.400±0.033)μmol/g,显著高于CYD-3-G对亚铁离子还原力(0.340±0.033)μmol/g(P<0.05);H2O2浓度为200μmol/L,处理6 h时,细胞存活率相较于空白组显著降低(P<0.05);ENPs-C3G复合物对H2O2诱导氧化损伤的HepG2细胞内ROS水平和NO质量摩尔浓度相较于HepG2氧化损伤模型均表现出显著降低现象(P<0.05),CAT和SOD酶活力水平相较于HepG2氧化损伤模型均表现为显著提高(P<0.05)。【结论】鳗鱼纳米粒子-花色苷复合物对H2O2诱导的HepG2细胞氧化损伤具有一定的保护作用。

外文摘要：【Objective】To explore the protective effect of eel(Muraenesox cinereus)nanoparticles on oxidative damage of HepG2 from human liver cancer cells,and establish the eel nanoparticles-anthocyanin delivery system.【Methods】Eel nanoparticle-anthocyanin complex(ENPs-C3G)delivery system was conducted to evaluate ABTS+radical scavenging activity and the ability to reduce FRAP.Furthermore,the HepG2 oxidative stress model was established using H2O2,the effects of the ENPs C3G complex on the H2O2-induced ROS,NO content,and SOD,as well as CAT activity in HepG2 cells were determined.【Results】The ABTS+radical scavenging activity of ENPS-C3G complex was significantly higher than that of CYD-3-G(P<0.05)and ENPS-C3G complex in 1∶20 group(material mass ratio)showed the strongest effect on the scavenging ABTS+·radicals,close to 100%.The FRAP reduction effect of ENPS-C3G was also stronger than that of CYD-3-G.The FRAP reduction effect of ENPS-C3G was(0.400±0.033)μmol/g in 1∶40 group,which was significantly higher than that of CYD-3-G with(0.340±0.033)μmol/g(P<0.05).Cell culture study revealed that when H_(2)O_(2)(concentration at 200μmol/L)treated HepG2 cells for 6 h,the cell viability was significantly decreased compared with the blank group(P<0.05).The levels of ROS and the content of NO in HepG2 cells induced by H_(2)O_(2)were significantly reduced by ENPS-C3G complex compared with oxidative damage model(P<0.05),the enzyme activities of CAT and SOD were significantly increased(P<0.05).【Conclusion】Eel nanoparticle-anthocyanin complex has a promising antioxidant activity in vitro and protective effect on H2O2-induced oxidative damage in HepG2 cells.