文献类型：期刊文献

英文题名：Molecular characterization and expression of interleukin-10 and interleukin-22 in golden pompano (Trachinotus ovatus) in response to Streptococcus agalactiae stimulus

作者：Peng Y.; Cai X.; Zhang G.; Wang J.; Li Y.; Wang Z.; Wang B.; Xiong X.; Wu Z.; Jian J.

机构：[1]Guangxi Key Laboratory of Marine Biotechnology, Guangxi Institute of Oceanology, Beihai, 536000, China;[2]Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Guangdong Ocean University, Zhanjiang, 524088, China

年份：2017

卷号：65

起止页码：244

外文期刊名：Fish and Shellfish Immunology

收录：Scopus(收录号：2-s2.0-85018331278)

语种：英文

外文关键词：Immune responses; Interleukin-10; Interleukin-22; Streptococcus agalactiae; Trachinotus ovatus

外文摘要：In the present study, members of the interleukin (IL)-10 family of cytokines, including IL-10 (TOIL-10) and IL-22 (TOIL-22) of golden pompano (Trachinotus ovatus), were cloned for the first time, and their expression patterns and 3D structures analyzed. The full-length cDNA sequences of TOIL-10 and TOIL-22 contained open reading frames of 564 and 567 bp, respectively. TOIL-10 and TOIL-22 shared higher homology (78%–89%) with the corresponding genes from various fish relative to other species (25%–34%) and contained the IL-10 family signature and four cysteine residues that are well conserved in other vertebrate IL-10 members. Phylogenetic tree analysis of our sequences alongside other IL-10 family proteins revealed that TOIL-10 and TOIL-22 cluster together with other teleost IL-10 and IL-22 molecules. Expression of TOIL-10 and TOIL-22 genes was ubiquitous in all tissues examined. The TOIL-10 gene was also highly expressed in skin, heart, gill, spleen, kidney, brain and liver, and lower levels were detected in intestine and muscle. High expression of the TOIL-22 gene was observed in gill, intestine, kidney, spleen, with the lowest levels in liver. TOIL-10 and TOIL-22 were rapidly activated after SAΔphoB immunization and significantly increased to peak levels at 12?h and 4?d in golden pompano kidney and spleen respectively following challenge. Expression in the brain reached peak levels at 4?d and 3?d respectively after post-immunization. Our results collectively indicate that TOIL-10 and TOIL-22 participate in the host immune response to bacterial infection. Moreover, TOIL-22 plays a potentially important role in mucosal immunity. ? 2017