文献类型：期刊文献

英文题名：SUMO-modified insulin-like growth factor 1 receptor (IGF-1R) increases cell cycle progression and cell proliferation

作者：Lin, Yingbo[1];Liu, Hongyu[1,2];Waraky, Ahmed[1];Haglund, Felix[1];Agarwal, Prasoon[3,4];Jernberg-Wiklund, Helena[3];Warsito, Dudi[1];Larsson, Olle[1]

机构：[1]Karolinska Inst, Dept Pathol & Oncol, CCK R8 04, Stockholm, Sweden;[2]Guangdong Ocean Univ, Fisheries Coll, Lab Aquat Anim Nutr Feed, Zhanjiang, Peoples R China;[3]Rudbeck Lab, Dept Immunol Genet & Pathol, Uppsala, Sweden;[4]Karolinska Univ Hosp, Div Clin Immunol, Dept Lab Med LABMED H5, Stockholm, Sweden

年份：2017

卷号：232

期号：10

起止页码：2722

外文期刊名：JOURNAL OF CELLULAR PHYSIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000407019900014)、、Scopus(收录号：2-s2.0-85018928333)、WOS

基金：Swedish Cancer Foundation, Grant number: CAN215/43; Swedish Research Council, Grant number: D0356401; Cancer Society in Stockholm, Grant number: 141232; Swedish Children Cancer Society, Grant number: PROJ12/020; National Natural Science Foundation of China, Grant number: 31272673; National Basic Research Programs of China, Grant number: 2014CB138600; Stockholm County Council and Karolinska Institutet

语种：英文

外文关键词：cancer; cell cycle; IGF-1R; proliferation; SUMOylation

外文摘要：Increasing number of studies have shown nuclear localization of the insulin-like growth factor 1 receptor (nIGF-1R) in tumor cells and its links to adverse clinical outcome in various cancers. Any obvious cell physiological roles of nIGF-1R have, however, still not been disclosed. Previously, we reported that IGF-1R translocates to cell nucleus and modulates gene expression by binding to enhancers, provided that the receptor is SUMOylated. In this study, we constructed stable transfectants of wild type IGF1R (WT) and triple-SUMO-site-mutated IGF1R (TSM) using igf1r knockout mouse fibroblasts (R-). Cell clones (R-WT and R-TSM) expressing equal amounts of IGF1R were selected for experiments. Phosphorylation of IGF-1R, Akt, and Erk upon IGF-1 stimulation was equal in R-WT and R-TSM. WT was confirmed to enter nuclei. TSM did also undergo nuclear translocation, although to a lesser extent. This may be explained by that TSM heterodimerizes with insulin receptor, which is known to translocate to cell nuclei. R-WT proliferated substantially faster than R-TSM, which did not differ significantly from the empty vector control. Upon IGF-1 stimulationG1-S-phase progression of R-WT increased from 12 to 38%, compared to 13 to 20% of R-TSM. The G1-S progression of R-WT correlated with increased expression of cyclin D1, A, and CDK2, as well as downregulation of p27. This suggests that SUMO-IGF-1R affects upstream mechanisms that control and coordinate expression of cell cycle regulators. Further studies to identify such SUMO-IGF-1R dependent mechanisms seem important.