文献类型：期刊文献

中文题名：两株对虾幼体弧菌病病原的分离和鉴定

英文题名：Isolation and Identification of Two Pathogenic Strains Associated with Vibriosis of Shrimp Larvae

作者：温崇庆[1];温崇[2];薛明[1];何红[3];周世宁[2]

机构：[1]广东海洋大学水产学院,湛江 524088;[2]中山大学有害生物控制与资源利用国家重点实验室,广州 510275;[3]广东海洋大学农学院,湛江 524088

年份：2008

卷号：35

期号：3

起止页码：346

中文期刊名：微生物学通报

外文期刊名：Microbiology China

收录：CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

基金：国家“863”项目资助(No.2003AA624010);广东省自然科学基金资助(No.05011780)

语种：中文

中文关键词：副溶血弧菌;溶藻弧菌;R72H序列;16S;rRNA基因;HSP60基因;鉴定

外文关键词：Vibrio parahaemolyticus, Vibrio alginolyticus, R72H sequence, 16S rRNA gene, HSP60 gene,Identification.

中文摘要：从患弧菌病的凡纳滨对虾(Litopenaeus vannamei)幼体中分离到两株病原菌zouA和zouB,常规形态和生理生化试验表明均为弧菌属菌种,弧菌编码鉴定系统分别鉴定为溶藻弧菌(Vibrio alginolyticus)和副溶血弧菌(V.parahaemolyticus)。副溶血弧菌R72H序列检测结果进一步证实菌株zouB为副溶血弧菌。对菌株zouA的16S rRNA基因序列分析表明该菌株与溶藻弧菌、副溶血弧菌等弧菌的相似性均高于98%,相互间不能区分;HSP60基因序列分析表明该菌株与溶藻弧菌相似性达98%以上,而与所有其它弧菌的相似性不到92%。结合表型和分子特征的鉴定结果,菌株zouA和zouB分别被鉴定为溶藻弧菌和副溶血弧菌。

外文摘要：Two pathogenic strains zouA and zouB were isolated from Litopenaeus vannamei larvae which suffered from vibriosis, and were identified as Vibrio alginolyticus and V. parahaemolyticus by traditional bacterial identification method respectively. Strain zouB was confirmed further as V. parahaemolyticus by special R72H sequence test. 16S rRNA and heat shock protein （HSP60） genes partial sequences of strain zouA were determined. Phylogenetic tree of vibrios based on 16S rRNA gene sequences revealed that there was more than 98% sequence identity among zouA, V. alginolyticus, V. parahaemolyticus, et al., and could not be distinguished between each other. HSP60 gene sequences showed that strain zouA had sequence identity of more than 98% to V. alginolyticus, whereas had less than 92% to that of all the other vibrios. Combining phenotypic and molecular characters, zouA and zouB were identified as V. alginolyticus and V. parahaemolyticus respectively.