文献类型：期刊文献

英文题名：Biodegradation of Di-(2-ethylhexyl) Phthalate by Rhodococcus ruber YC-YT1 in Contaminated Water and Soil

作者：Yang, Ting[1];Ren, Lei[2];Jia, Yang[1];Fan, Shuanghu[1];Wang, Junhuan[1];Wang, Jiayi[1];Nahurira, Ruth[1];Wang, Haisheng[1];Yan, Yanchun[1]

机构：[1]Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China;[2]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Peoples R China

年份：2018

卷号：15

期号：5

外文期刊名：INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH

收录：SSCI(收录号：WOS:000435197300132)、SCI-EXPANDED(收录号：WOS:000435197300132)、、Scopus(收录号：2-s2.0-85046857244)、WOS

基金：We thank, Bisheng Wang (Institute of Agricultural Resources and Regional Planning, CAAS, China) for measuring the physicochemical properties of the soil. We also acknowledge Lida Han (Biotechnology Research Institute of CAAS, China) for great help and good suggestions for HPLC-MS analysis. This work was supported by the National Natural Science Foundation of China (Nos. 31170119, 31540067) and the Basic Research Fund of CAAS (Nos. 0042014011, 1610042017001).

语种：英文

外文关键词：biodegradation; di-(2-ethylhexyl) phthalate; Rhodococcus ruber; marine plastic debris; bioremediation

外文摘要：Di-(2-ethylehxyl) phthalate (DEHP) is one of the most broadly representative phthalic acid esters (PAEs) used as a plasticizer in polyvinyl chloride (PVC) production, and is considered to be an endocrine-disrupting chemical. DEHP and its monoester metabolites are responsible for adverse effects on human health. An efficient DEHP-degrading bacterial strain Rhodococcus ruber YC-YT1, with super salt tolerance (0-12% NaCl), is the first DEHP-degrader isolated from marine plastic debris found in coastal saline seawater. Strain YC-YT1 completely degraded 100 mg/L DEHP within three days (pH 7.0, 30 degrees C). According to high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis, DEHP was transformed by strain YC-YT1 into phthalate (PA) via mono (2-ethylehxyl) phthalate (MEHP), then PA was used for cell growth. Furthermore, YC-YT1 metabolized initial concentrations of DEHP ranging from 0.5 to 1000 mg/L. Especially, YC-YT1 degraded up to 60% of the 0.5 mg/L initial DEHP concentration. Moreover, compared with previous reports, strain YC-YT1 had the largest substrate spectrum, degrading up to 13 kinds of PAEs as well as diphenyl, p-nitrophenol, PA, benzoic acid, phenol, protocatechuic acid, salicylic acid, catechol, and 1,2,3,3-tetrachlorobenzene. The excellent environmental adaptability of strain YC-YT1 contributed to its ability to adjust its cell surface hydrophobicity (CSH) so that 79.7-95.9% of DEHP-contaminated agricultural soil, river water, coastal sediment, and coastal seawater were remedied. These results demonstrate that R. ruber YC-YT1 has vast potential to bioremediate various DEHP-contaminated environments, especially in saline environments.