文献类型：期刊文献

英文题名：Use of molecular biomarkers to estimate manganese requirements for broiler chickens from 22 to 42 d of age

作者：Lu, Lin[1];Chang, Bin[1,2];Liao, Xiudong[1];Wang, Runlian[2];Zhang, Liyang[1];Luo, Xugang[1]

机构：[1]Chinese Acad Agr Sci CAAS, Inst Anim Sci, Mineral Nutr Res Div, Beijing 100193, Peoples R China;[2]Guangdong Ocean Univ, Dept Anim Sci, Zhanjiang 524088, Peoples R China

年份：2016

卷号：116

期号：9

起止页码：1512

外文期刊名：BRITISH JOURNAL OF NUTRITION

收录：SCI-EXPANDED(收录号：WOS:000388352600003)、、Scopus(收录号：2-s2.0-84994104674)、WOS

基金：The present study was supported by the Key International Cooperation Program of the National Natural Science Foundation of China (grant no. 31110103916; Beijing), the Agricultural Science and Technology Innovation Program (grant no. ASTIP-IAS08; Beijing) and the China Agriculture Research System (grant no. CARS-42; Beijing).

语种：英文

外文关键词：Gene expression; Manganese; Manganese-containing superoxide dismutase; Dietary requirements; Broilers

外文摘要：The present study was carried out to evaluate dietary Mn requirements of broilers from 22 to 42 d of age using molecular biomarkers. Chickens were fed a conventional basal maize-soyabean meal diet supplemented with Mn as Mn sulphate in graded concentrations of 20 mg Mn/kg from 0 to 140 mg Mn/kg of diet for 21 d (from 22 to 42 d of age). The Mn response curves were fitted for ten parameters including heart Mncontaining superoxide dismutase (MnSOD) mRNA and its protein expression levels and the DNA-binding activities of specificity protein 1 (Sp1) and activating protein-2 (AP-2). Heart MnSOD mRNA and protein expression levels showed significant quadratic responses (P < 0.01), and heart MnSOD activity showed a broken-line response (P < 0.01), whereas Mn content and DNA-binding activities of Sp1 and AP-2 in the heart displayed linear responses (P < 0.01) to dietary Mn concentrations, respectively. The estimates of dietary Mn requirements were 101, 104 and 94 mg/kg for full expressions of MnSOD mRNA level, MnSOD protein level and MnSOD activity in the heart, respectively. Our findings indicate that heart MnSOD mRNA expression level is a more reliable indicator than heart MnSOD protein expression level and its activity for the evaluation of Mn requirement of broilers, and about 100 mg Mn/kg of diet is required for the full expression of heart MnSOD in broilers fed the conventional basal maize-soyabean meal diet from 22 to 42 d of age.