文献类型：期刊文献

英文题名：Dietary sanguinarine supplementation on the growth performance, immunity and intestinal health of grass carp (Ctenopharyngodon idellus) fed cottonseed and rapeseed meal diets

作者：Liu, Yan-Li[1];Zhong, Lei[1,2];Chen, Tuan[1];Shi, Yong[1];Hu, Yi[1,2];Zeng, Jian-Guo[2];Liu, Hong-Yu[3];Xu, Shu-De[4]

机构：[1]Hunan Agr Univ, Hunan Engn Res Ctr Utilizat Characterist Aquat Re, Changsha 410128, Hunan, Peoples R China;[2]Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha 410128, Hunan, Peoples R China;[3]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Anim Nutr & Feed, Zhanjiang 524088, Guangdong, Peoples R China;[4]Guangdong VTR Biotech Co Ltd, Zhuhai 519060, Guangdong, Peoples R China

年份：2020

卷号：528

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000553682000006)、、WOS

基金：This study was supported by Key Research and Development Project in Hunan province (2017NK2302), Hunan provincial natural science foundation of China (2020JJ4044), and Major Science and Technology Project of Hunan province (2017NK1030). The authors would like to thank the personnel of these teams for their kind assistance.

语种：英文

外文关键词：Ctenopharyngodon idellus; Sanguinarine; Immune response; Intestinal morphology; Intestinal microbiota

外文摘要：Sanguinarine (SG) is a plant alkaloid with demonstrated anti-inflammatory, antioxidant, and antimicrobial properties. However, its effects on the immunity and intestinal health of grass carp have not been clarified. Herein, an eight-week feeding trial was conducted to evaluate the effects of SG on the growth, immunity, and intestinal health of grass carp (Ctenopharyngodon idellus). A basal diet of 5% fish meal and 44% cottonseed and rapeseed meal was designated as the control diet (FM group). Diets (2% fish meal, 55% cottonseed and rapeseed meal) supplemented with four SG concentrations (0, 0.2, 0.4, and 0.8 g.kg(-1); designated as the SGO, SG0.2, SG0.4, and SG0.8 groups, respectively) were prepared. The results indicate that dietary SG in high cottonseed and rapeseed meal diets increased the survival rate (SR), weight gain rate (WGR), superoxide dismutase (SOD), and alkaline phosphatase (AKP) activities compared with the SGO group, but not significantly. The activity of malondialdehyde (MDA) was significantly lower in the SG0.2-0.8 groups than SGO group. The SGO group had a significantly decreased number of goblet cells and villus height/crypt depth (V/C), significantly downregulated mRNA expression levels of MnSOD, claudin, occludin, ZO-1, and ZO-2, and significantly upregulated mRNA expression levels of TNF-alpha, IL-1 beta, TLR-7, and TLR-8 compared with those in the FM group. While the number of goblet cells and V/C were significantly greater, the expression of MnSOD, ZO-1, and ZO-2 was significantly upregulated and the mRNA expression levels of TNF-alpha, IL-1 beta, IL-6, TLR-4, TLR-7, and TLR-8 were significantly downregulated in the SG0.8 group compared with those in the SGO group. The proportion of Aeromonas significantly increased, and the Bacteroides significantly decreased in the SGO group compared with the FM group. However, the proportion of Aeromonas showed significantly decreased, while the proportion of Bacteroides increased in the SG0.8 group compared with the SGO group. Furthermore, based on microbial function, the gut microbiota of the SG0.8 group were more active concerning the energy, lipid, and amino acid metabolisms compared with those of the SGO group. In conclusion, the feed with a higher cottonseed and rapeseed meal concentration negatively affects the SR, intestinal structure and immunity of grass carp. However, dietary SG can improve the SR and WGR by enhancing the intestinal morphology, microbiota structure, and mRNA expression levels of genes related to intestinal immunity.