文献类型：期刊文献

中文题名：猪各组织器官内肌生成抑制素基因mRNA表达谱的研究

英文题名：Studies on the expression pattern of myostatin mRNA in porcine tissues

作者：张锐[1];孙美榕[2];张红莲[1];杨捷[1];黄燕[1];陈绍红[1];杜慧[1];欧阳红生[3]

机构：[1]湛江海洋大学现代生化中心,广东湛江524088;[2]深圳科安信公司,广东深圳518057;[3]中国人民解放军军需大学生物化学与分子生物学教研室,吉林长春130062

年份：2004

卷号：38

期号：3

起止页码：16

中文期刊名：中国兽药杂志

外文期刊名：Chinese Journal of Veterinary Drug

收录：CSTPCD

语种：中文

中文关键词：猪;组织器官;肌生成抑制素;基因表达;mRNA;表达谱;肌肉生成;负调控因子

外文关键词：porcine myostatin gene; mRNA; expression pattern

中文摘要：　用Trizol试剂法提取军牧一号猪的骨骼肌、心肌、肝、肾、肺、脾、胰、脂肪的总RNA做RT-PCR和嵌套PCR,结果在军牧一号猪骨骼肌、心肌和脂肪组织内检测到了肌生成抑制素mRNA的表达,而在其他组织中未检测到。

外文摘要：Myostatin is a negative regulator of muscle growth. It can be expressed in skeletal muscle, galactophore and cardiomyocytes. In order to investgate the expression pattern of myostatin mRNA in others tissues, total RNAs were isolated from skeletal muscle, cardiac muscle, liver, kidney, lung, spleen, pancreas, and fat cells of a new pig breed JunMu-1 with Trizol reagent. Using RT-PCR and a nested PCR, it was found that the expression of mRNAs were detected in skeletal muscle, cardiac muscle and fat, and not detected in the other tissues. The result was coincident with the published literatures.