文献类型：期刊文献

英文题名：Effects of fish oil on ovarian development in spotted scat (Scatophagus argus)

作者：Zhang, Min-Zhi[1,2];Li, Guang-Li[1,2];Zhu, Chun-Hua[1,2];Deng, Si-Ping[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Anim Guangdong Higher Educ Inst, Key Lab Aquaculture South China Sea Aquat Econ, Zhanjiang 524088, Peoples R China

年份：2013

卷号：141

期号：1-2

起止页码：90

外文期刊名：ANIMAL REPRODUCTION SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000324451000012)、、Scopus(收录号：2-s2.0-84883054617)、WOS

基金：This study was supported by the National Natural Science Foundation of China (No. 31272640). Special thanks to Zhuhai Longsheng Fry Cultivation Base (Zhuhai, Guangdong, PR China) for rearing the fish, Dr. Mei-Yi Xu for sex steroid analysis and Dr. Qi-Hui Yang for preparing diets. We gratefully acknowledge Dr. Ya-Xiong Tao (Auburn University, Auburn, AL, USA) for expert editing of the manuscript.

语种：英文

外文关键词：Scatophagus argus; Fish oil; Ovarian development; Sex steroid hormone; Serum protein; vtg expression

外文摘要：The effects of different concentrations of dietary fish oil (0, 2%, or 6%) on ovarian development in 2-year-old female Scatophagus argus were investigated. The levels of serum sex steroid hormones (estradiol-17 beta, E-2; testosterone, T), protein phosphorus (SPP), and protein calcium (SPC), as well as vitellogenin (vtg) mRNA expression in livers and ovaries were measured. Over the eight week experimental period, oocytes did not develop further and remained at phase III in fish fed with the control diet with no supplement of fish oil. Fish fed with 2% fish oil supplement had oocytes at transition phase from III to IV. Fish fed with 6% fish oil supplement had oocytes at late phase IV. Higher gonadosmatic index, serum E-2, SPP, SPC, and liver vtg expression were found in 6% fish oil group compared to that in the 2% fish oil group (except E-2) and the control group (P<0.05). In addition, vtg expression in livers was 600-1000 times higher than that in the ovaries. Gonadosmatic index, E-2, and SPP, as well as liver vtg expression increased during the experiment and peaked at the end of experiment. However, hepatosomatic index, serum T, and ovarian vtg expression peaked at 4 weeks, and then decreased at 8 weeks, with no significant difference among the 3 groups. In summary, we showed that 6% fish oil supplementation in S. argus could effectively promote ovarian development, with associated increases in E-2 secretion and increased liver vtg mRNA expression. (C) 2013 Elsevier B.V. All rights reserved.