文献类型：期刊文献

中文题名：我国南粤地区霉变水产饲料真菌毒素污染现状及毒性评价

英文题名：Mycotoxins contamination situation and toxicity evaluation of moldy aquatic feed in southern Guangdong of China

作者：王小博[1];王雅玲[1];王润东[1];吕鹏莉[1];孙力军[1];刘晓燕[1];施琦[2];毕思远[3]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东普通高等学校水产品深加工重点实验室,广东湛江524088;[2]湛江市质量计量监督检测所,广东湛江524022;[3]深圳市通量检测科技有限公司,广东深圳518012

年份：2016

卷号：28

期号：6

起止页码：951

中文期刊名：浙江农业学报

外文期刊名：Acta Agriculturae Zhejiangensis

收录：CSTPCD、、北大核心2014、CSCD2015_2016、北大核心、CSCD

基金：国家自然科学基金项目(31171634,31371777);广东省科技计划项目(2014B020205006);广东省渔业局项目(粤财农[2015]115号);广东省高等学校创新强校项目(GDOU2013050312,GDOU2013050205,GDOU2013050203);广东省质量技术监督局科技项目;深圳市技术研究与开发基金项目(GCZX20140509163150112737)

语种：中文

中文关键词：水产饲料;霉变;真菌毒素;污染调查;毒性评价

外文关键词：aquatic feed;mildew;mycotoxins;pollution survey;toxicity evaluation

中文摘要：为了解广东地区水产饲料中真菌毒素的最新污染状况,评价霉变水产饲料的毒性效应,探明其对生物机体的损害效应。在我国南粤地区随机采集鱼、虾饲料共120份,采用ELISA法检测4种真菌毒素,黄曲霉毒素B1(AFB1)、T-2毒素、呕吐毒素(DON)、赭曲霉毒素(OTA)的含量,选取不同污染程度(达标、轻度、中度和重度)的鱼、虾饲料各4份,采用小鼠毒理学方法评价其生物毒性。结果发现:水产饲料真菌毒素污染率较高(多种毒素并存现象较为普遍),其中AFB1,T-2,DON等3种毒素污染最为普遍,OTA污染较轻,T-2检测浓度最大(11 928.00μg·kg^(-1));小鼠体质量呈先上升后下降趋势,各器官指数略有下降,脾、肾下降较为明显;淋巴细胞、血红蛋白及大血小板比率显著下降(P<0.05);精子畸形率和微核率随剂量增加而升高,血液生化指标差异显著(P<0.05)。广东地区霉变水产饲料真菌毒素污染严重,T-2含量最高;霉变饲料对小鼠脾、肾、肝器官损害显著,对小鼠造血系统、免疫系统具有损害作用,并具有遗传毒性。

外文摘要：In order to understand the latest mycotoxin contamination situation of aquatic feed and raw materials in Guangdong Province, evaluate toxic effect of moldy aquatic feed, and understand their damaging effects on living or-ganisms, a total of 120 samples were randomly collected from the fish and shrimp feed in the south of Guangdong. Enzyme linked immunosorbent assay （ ELISA） was used to detect the contents of mycotoxins including aflatoxin B1 （ AFB1 ） , T-2 toxin, deoxynivalenol （ DON） and ochratoxin （ OTA） in above aquatic feeds, and then each four fish feed and shrimp feed with different mycotoxin pollution levels （ standard, mild, moderate and severe） were chosen to evaluate biological toxicity for mouse. The results showed that the mycotoxin contamination rate in aquatic feed was high, coexisting phenomenon of several toxins was relatively common; And T-2, DON, AFB1 toxin pollutions were the most common, OTA pollution was lighter, T-2 detected concentration was the largest （ 11928. 00 μg·kg^-1 ） . Mice weight increased first then fell, each organ index fell slightly, especially the spleen and kidney indexes. Lym-phocyte, hemoglobin, the large platelet rate were decreased significantly （P〈0. 05）. Sperm deformity rate and mi-cronucleus rate increased with the increasing dose, and significant differences were found in the blood biochemical indexes （P〈0. 05）. The study showed that mycotoxin contamination was serious and T-2 content was the highest in moldy aquatic feed, which had significant damage on mice spleen, kidney and liver organs. Meanwhile, it also had adverse effects on the hematopoietic and immune systems, and had genetic toxicity at certain level.