文献类型：期刊文献

英文题名：Orientin Induces G0/G1 Cell Cycle Arrest and Mitochondria Mediated Intrinsic Apoptosis in Human Colorectal Carcinoma HT29 Cells

作者：Thangaraj, Kalaiyarasu[1,2];Balasubramanian, Balamuralikrishnan[3];Park, Sungkwon[3];Natesan, Karthi[2,4];Liu, Wenchao[5];Manju, Vaiyapuri[2]

机构：[1]Bharath Inst Higher Educ & Res, Dept Microbiol & Biotechnol, Chennai 600045, Tamil Nadu, India;[2]Periyar Univ, Dept Biochem, Mol Oncol Lab, Salem 636011, Tamil Nadu, India;[3]Sejong Univ, Coll Life Sci, Dept Food Sci & Biotechnol, Seoul 05006, South Korea;[4]RDA, Natl Acad Agr Sci, Genom Div, Jeollabuk 560500, South Korea;[5]Guangdong Ocean Univ, Coll Agr, Dept Anim Sci, Zhanjiang 524088, Peoples R China

年份：2019

卷号：9

期号：9

外文期刊名：BIOMOLECULES

收录：SCI-EXPANDED(收录号：WOS:000489102800030)、、Scopus(收录号：2-s2.0-85071622397)、WOS

基金：This research received no external funding. The authors were grateful to the authorities of Periyar University for the necessary facilities to carry out this research work and thank the Sejong University, Republic of Korea for their support.

语种：英文

外文关键词：colorectal cancer; orientin; cell cycle arrest; Bcl-2 family proteins; apoptosis

外文摘要：Colorectal carcinoma is one of the utmost diagnosed cancer with a steep increase in mortality rate. The incidence has been increasing in developing countries like India due to a westernization life style. Flavonoids have been explored widely for its various pharmacological activity including antitumor activity. Orientin, an analogue of luteolin (citrus flavonoid) isolated from rooibos and tulsi leaves is also expected to deliver significant antitumor activity similar to that of luteolin. The present study anticipates exploring the antitumor activity of orientin against colorectal carcinoma cells (HT29). Orientin exhibited remarkable cytotoxicity and antiproliferative activity against HT29 cells, which is clearly evident from tetrazolium based cytotoxicity and lactate dehydrogenase release assays. Orientin induce G0/G1 cell cycle arrest and regulates cyclin and cyclin-dependent protein kinases in order to prevent the entry of the cell cycle to the S phase. Annexin V-FITC (V-Fluorescein Isothiocyanate) dual staining reveals the apoptotic induction ability of orientin. The Bcl-2 family proteins along with the inhibitor of apoptotic proteins were regulated and the tumor suppressor p-53 expression have been decreased. In conclusion, our results proposed that orientin could be a potent chemotherapeutic agent against colorectal cancer after ascertaining their molecular mechanisms.