文献类型：期刊文献

英文题名：Structural Characterization and Antioxidant Activity Mechanism of the Feruloylated Neutral Sugar Side Chain from Sugar Beet Pectin

作者：Xiang, Chunhong[1]; Teng, Hui[1]; Sheng, Zhili[2]; Zhao, Chao[3]; Deng, Jingteng[1]; Zhao, Chengang[1]; He, Bo[1]; Chen, Lei[1]; Ai, Chao[1]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang, 524088, China; [2] Guangdong Provincial Key Laboratory of Applied Botany, Chinese Academy of Sciences, South China Botanical Garden, Guangzhou, 510650, China; [3] College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

年份：2024

外文期刊名：SSRN

收录：EI(收录号：20240242542)

语种：英文

外文关键词：Alkylation - High performance liquid chromatography - Nuclear magnetic resonance - Sugar beets

外文摘要：Feruloylated neutral sugar side chains are thought to play a crucial role in the biological effects of sugar beet pectin. In this study, SBP-3A, a novel feruloylated polysaccharide fragment, was isolated from sugar beet pectin utilizing enzymatic digestion. The presence of ferulic acid on SBP-3A was confirmed through high-performance liquid chromatography (HPLC), with a mass fraction of 22.5 μg/mg. The average molecular weight was determined to be 33.31 kDa. Methylation analysis, and nuclear magnetic resonance (NMR) spectra revealed that SBP-3A is a heteroglycan with the main chain structure of →2)-α-Rhap-(1→4)-α-GalpA-(1→2)-α-Rhap-(1→, and the branched chain structure of ferulic acid (FA) →3,4)-β-Galp-(1→2,4)-α-Rhap-(1→. Subsequently, the antioxidant activity of SBP-3A was evaluated using the Caenorhabditis elegans (C. elegans). SBP-3A improved antioxidant enzymes and non-enzymatic defense system, decreased reactive oxygen species levels, and up-regulated the mRNA expression of sod-3, skn-1, and daf-16, while down-regulated the expression of age-1 in C. elegans. Moreover, SBP-3A modulated the gut flora by favorably affecting the abundances of Lactobacillus, Ligilactobacillus, and Akkermansia, thereby enhancing antioxidant capacity in C. elegans. Consequently, the aforementioned findings support the potential application of SBP-3A as a functional food for treating oxidative stress-related illnesses. ? 2024, The Authors. All rights reserved.