文献类型：期刊文献

英文题名：Preparation and Structure Characterization of High-Value Laminaria digitata Oligosaccharides

作者：Cheong, Kit-Leong[1,2];Li, Jia-Kang[1,2];Zhong, Saiyi[1]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China;[2]Shantou Univ, Coll Sci, Dept Biol, Shantou, Peoples R China

年份：2022

卷号：9

外文期刊名：FRONTIERS IN NUTRITION

收录：SCI-EXPANDED(收录号：WOS:000829819900001)、、WOS

基金：Funding This research was financially supported by Key-Area Research and Development Program of Guangdong Province (2020B1111030004), the Innovative Team Program of High Education of Guangdong Province (2021KCXTD021), and the Special Project of Guangdong Science and Technology Department (210718205862114).

语种：英文

外文关键词：laminaran; oligosaccharide; Laminaria digitata; size exclusion chromatography; biological activity

外文摘要：Algae-derived marine oligosaccharides have been reported to be promising bioactive compounds because of their various properties with health benefits and potential significance in numerous applications in industrial biotechnology. In this study, laminaran oligosaccharides (LOs) with varying degrees of polymerization were obtained through partial acid hydrolysis of laminaran derived from Laminaria digitata. Based on response surface methodology, the optimum LOs yield was obtained for acid hydrolysis laminaran at a hydrolysis time of 55 min, temperature of 71 degrees C, and acid concentration of 1.00 mol/L. The size-exclusion resin Bio-Gel P-2 was considered to be a better option for LOs purification. The structure of the purified oligosaccharides was analyzed through mass spectrometry and nuclear magnetic resonance. They demonstrated the main oligosaccharide structure corresponding to the connection of glucose with beta-D-Glcp-(1 -> 3)-beta-D-Glcp, which was identified as laminaribiose (DP2), laminaritriose (DP3), laminaritetrose (DP4), and laminaripentaose (DP5). LOs demonstrate excellent antioxidant activities, as evidenced from their reactions with oxidizing free radicals, 1, 1-diphenyl-2-picryl-h(y)drazyl, and 2, 2'-azino-bis (3-etilbenzotiazoline-6-sulfonic acid) radicals. LOs exhibited a prebiotic effect on the growth of Bifidobacterium adolescentis and Lactobacillus plantarum. Therefore, we propose the development of LOs as natural antioxidants and prebiotics in the functional food and pharmaceutical industries.