文献类型：期刊文献

英文题名：Monitoring the contents of six steroidal and phenolic endocrine disrupting chemicals in chicken, fish and aquaculture pond water samples using pre-column derivatization and dispersive liquid-liquid microextraction with the aid of experimental design methodology

作者：Wu, Hongliang[1,2,3];Li, Guoliang[1];Liu, Shucheng[4];Hu, Na[2,3];Geng, Dandan[2,3];Chen, Guang[1];Sun, Zhiwei[1];Zhao, Xianen[1];Xia, Lian[1];You, Jinmao[1,2]

机构：[1]Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China;[2]Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China;[3]Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China;[4]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China

年份：2016

卷号：192

起止页码：98

外文期刊名：FOOD CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000362304500013)、、EI(收录号：20152801017909)、Scopus(收录号：2-s2.0-84936805005)、WOS

基金：This work was supported by The National Natural Science Foundation of China (Nos. 31301595, 21475074, 21275089, 21305076 and 81303179), Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety (GDPKLAPPS1401), and PhD research start-up funds of Qufu Normal University (bsqd 2012017).

语种：英文

外文关键词：Endocrine disrupting chemicals; Dispersive liquid-liquid microextraction; Pre-column derivatization; High-performance liquid chromatography with fluorescence detection; Box-Behnken design

外文摘要：This research established a sensitive and efficient pre-column derivatization HPLC method based on dispersive liquid-liquid microextraction (DLLME) for the simultaneous determination of six steroidal and phenolic endocrine disrupting chemicals (EDCs). In this study, EDCs were firstly labeled by the derivatization reagent 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) and then extracted by DLLME. The response surface methodology was employed to investigate the key parameters of pre-column derivatization and DLLME. Under the optimal conditions, a good linear relationship between the peak area and the concentration of analytes was observed with correlation coefficients of >0.9991. Limits of detection for all EDCs derivatives were achieved within the range of 0.02-0.07 mu g L-1. The proposed method has the advantages of simple operation, low consumption of organic solvent, saving time, low output limit and good selectivity. When applied to several food and water samples analysis, it demonstrated good applicability for the determination of EDCs. (C) 2015 Elsevier Ltd. All rights reserved.