文献类型：期刊文献

中文题名：姜黄素超分子包合物对乙醇诱导LO2细胞损伤的保护作用

英文题名：Protective Effect of Curcumin/Cyclodextrin Polymer Inclusion Complex on LO2 Cells Damaged by Ethanol

作者：范土贵[1,2];陈建平[1,2];高加龙[1,2];钟赛意[1,2];秦小明[1,2]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东省海洋生物制品工程实验室,水产品深加工广东普通高等学校重点实验室,广东省亚热带果蔬加工科技创新中心,广东湛江524088;[2]大连工业大学,海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034

年份：2021

卷号：42

期号：18

起止页码：366

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD、、EI(收录号：20241015701745)、北大核心、北大核心2020

基金：广东省自然科学基金面上项目(2020A1515010860,2021A1515012455);广东海洋大学创新强校项目(230419100);广东海洋大学“南海学者计划”项目(002029002009)。

语种：中文

中文关键词：姜黄素超分子包合物;LO2;细胞;乙醇损伤;保护作用;活性氧

外文关键词：curcumin/cyclodextrin polymer inclusion complex;LO2 cells;ethanol-induced injury;protective effect;reactive oxygen species

中文摘要：本文考察了姜黄素超分子包合物(Curcumin/Cyclodextrin polymer inclusion complex,CUR/CDP)对乙醇诱导LO2细胞损伤的保护作用。采用MTT法建立乙醇诱导LO2细胞损伤的模型,通过谷氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPX)、丙二醛(MDA)和活性氧(ROS)等试剂盒考察了CUR/CDP对乙醇诱导LO2细胞损伤的保护效果。结果表明,经CUR/CDP(80μg/mL)处理后,LO2细胞的存活率从54.75%±8.97%(模型对照组)提高到85.27%±2.64%,且细胞培养液中ALT、AST和LDH活力分别从(26.47±0.90)、(41.02±4.41)、(63.77±4.95)U/L(模型对照组)降低到(16.17±0.42)、(22.62±0.79)、(32.25±1.69)U/L(P<0.01),LO2细胞内GSH-PX、SOD活力分别从(21.82±1.34)、(8.45±1.11)U/mg prot(模型对照组)升高到(36.70±0.56)、(16.47±1.27)U/mg prot,LO2细胞内MDA和ROS含量分别从(1.19±0.15)nmol/mg prot、(198.02%±11.76%)(模型对照组)降低到(0.72±0.05)nmol/mg prot、(110.87%±10.22%)(P<0.01)。综上所述,CUR/CDP通过提高LO2细胞相关抗氧化酶的活力和降低细胞内ROS含量来改善乙醇诱导LO2细胞的损伤。

外文摘要：This study investigated the protective effect of curcumin/cyclodextrin polymer inclusion complex(CUR/CDP)on LO2 cells damaged by ethanol.The MTT method was used to establish a model of LO2 cells damaged by ethanol.Glutamate alanine aminotransferase(ALT),aspartate aminotransferase(AST),lactate dehydrogenase(LDH),superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),malondialdehyde(MDA)and reactive oxygen species(ROS)kits were used to investigate the protective effect of CUR/CDP on LO2 cells damaged by ethanol.The results showed that after CUR/CDP(80μg/mL)treatment,the cell activity of LO2 cells increased from(54.75%±8.97%)(model group)to 85.27%±2.64%,and the activities of ALT,AST,and LDH in the LO2 cell culture medium decreased from(26.47±0.90),(41.02±4.41),(63.77±4.95)U/L(model group)to(16.17±0.42),(22.62±0.79),(32.25±1.69)U/L(P<0.01),respectively;the activities of GSH-PX and SOD in LO2 cells increased from(21.82±1.34),(8.45±1.11)U/mg prot(model group)to(36.70±0.56),(16.47±1.27)U/mg prot;the content of MDA and ROS in LO2 cells decreased from(1.19±0.15)nmol/mg prot,(198.02%±11.76%)(model group)to(0.72±0.05)nmol/mg prot,(110.87%±10.22%)(P<0.01),respectively.In conclusion,CUR/CDP could improve the damage of LO2 cells induced by ethanol by increasing the activity of antioxidant enzymes and reducing the content of ROS.