文献类型：期刊文献

英文题名：The progestin norethindrone alters growth, reproductive histology and gene expression in zebrafish (Danio rerio)

作者：Liang, Yan-Qiu[1];Jing, Zhanxin[1];Pan, Chang-Gui[2];Lin, Zhong[1];Zhen, Zhen[3];Hou, Liping[4];Dong, Zhongdian[5]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Guangxi Univ, Guangxi Lab Study Coral Reefs South China Sea, Nanning 530004, Peoples R China;[3]Guangdong Ocean Univ, Agr Coll, Zhanjiang 524088, Peoples R China;[4]Guangzhou Univ, Sch Life Sci, Guangzhou 510655, Peoples R China;[5]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China

年份：2020

卷号：242

外文期刊名：CHEMOSPHERE

收录：SCI-EXPANDED(收录号：WOS:000509786600066)、、EI(收录号：20194507627387)、Scopus(收录号：2-s2.0-85074355254)、WOS

基金：The authors would like to acknowledge the financial support from the National Natural Science Foundation of China (41907346 and 41806195), Natural Science Foundation of Guangdong Province (2019A1515011889, 2017A030310662, 2018A030313131 and 2018A030307054), and PhD Start Fund of Guangdong Ocean University (R17075). Thanks also to the six anonymous reviewers for their useful comments and suggestions.

语种：英文

外文关键词：Progestin; Norethindrone; Endocrine disruption; Gene transcription; Fish

外文摘要：The aim of this study was to assess the long-term effects of synthetic progestin norethindrone (NET) on the growth, reproductive histology, and transcriptional expression profiles of genes associated with the hypothalamic-pituitary-gonadal (HPG) axis and germ cells in adult zebrafish. Adult zebrafish were exposed to 7, 84 and 810 ng/L NET for 90 days. The results showed that exposure to 810 ng/L NET caused a significant decrease in growth of females and males. The ovary weight and GSI was significantly reduced by NET at concentrations of 84 or 810 ng/L, which came along with the delay of ovary maturation in females. However, NET at all treatments resulted in acceleration of sperm maturation in males. In the ovaries of females, a strong inhibition of cyp19a1a gene was observed following exposure to NET at 810 ng/L. Similarly, NET at the highest treatment led to a significant down-regulation of cyp17, cyp19a1a, vasa, nanos1, dazl and dmc1 genes in the testes of males. Taken together, the overall results demonstrated that NET could impact growth and gonadal maturation, with significant alterations of transcriptional expression genes along HPG axis and germ cells. (C) 2019 Elsevier Ltd. All rights reserved.