文献类型：期刊文献

英文题名：A simple method for detecting and quantifying microplastics utilizing fluorescent dyes - Safranine T, fluorescein isophosphate, Nile red based on thermal expansion and contraction property

作者：Lv, Lulu[1,2];Qu, Junhao[3];Yu, Zihua[3];Chen, Daihuan[3];Zhou, Chunxia[2,3];Hong, Pengzhi[2,3,4];Sun, Shengli[1];Li, Chengyong[1,2,4]

机构：[1]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Guangdong, Peoples R China;[3]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[4]Guangdong Modern Agr Sci & Technol Innovat Ctr, Zhanjiang 524088, Peoples R China

年份：2019

卷号：255

外文期刊名：ENVIRONMENTAL POLLUTION

收录：SCI-EXPANDED(收录号：WOS:000498340400057)、、EI(收录号：20194007486208)、Scopus(收录号：2-s2.0-85072706324)、WOS

基金：This work was supported by the National Natural Science Foundation of China (21874029), Science and Technology Planning Project of Zhanjiang City (2018A02014), Guangdong Yangfan Program (201635018), Guangdong Special Support Program (2017TQ04N706), Science and Technology Planning Project of Shenzhen City (JCYJ20170818111719650), Industrial Development Special Funds of Dapeng New Area (KY20170209), Innovation and Development Project about Marine Economy Demonstration of Zhanjiang City (2017C8B1).

语种：英文

外文关键词：Microplastics; Staining; Thermal expansion and contraction; Identification; Quantification

外文摘要：Microplastics (particle size <5 mm) are an emerging contaminant for aquatic environmental, which have attracted increasing attention in worldwide range. In this study, an improved fluorescent staining method for detection and quantification of microplastics was developed based on thermal expansion and contraction. This method is effective in detection of polyethylene, polystyrene, polyvinyl chloride and polyethylene terephthalate plastic particles. In order to avoid error statistics caused by pretreatment, various characterizations of microplastics were measured after heated, such as microstructure, compositions and thermostability. The results showed that there was no significant damage to microplastics even under heating condition at 75 degrees C for 30 min, and the stained microplastics had strong stability for up to two months. Moreover, this method has been successfully applied to the quantification of microplastics in biological samples and result showed there were about 54 particles g(-1) (dry weight) microplastics in the Sipunculus nudus. This new method provides a reliable method for quantitative analysis of microplastics in environment and biological tissue. (C) 2019 Elsevier Ltd. All rights reserved.