文献类型：期刊文献

英文题名：Functional annotation and complete genome analysis confirm the probiotic characteristics of Bacillus species isolated from the gut of Nile tilapia

作者：Kuebutornye, Felix Kofi Agbeko[1,2]; Lu, Yishan[1,3,4,5,6,7]; Wang, Zhiwen[1,3,4,5,6,7]; Mraz, Jan[2]

机构：[1] Shenzhen Institute of Guangdong Ocean University, Guangdong, Shenzhen, 518120, China; [2] University of South Bohemia in ?eské Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Institute of Aquaculture and Protection of Waters, Na Sádkách 1780, ?eské Budějovice, 370 05, Czech Republic; [3] College of Fisheries, Guangdong Ocean University, Huguang Yan East, Guangdong, Zhanjiang, 524088, China; [4] Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Animals, Zhanjiang, 524088, China; [5] Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, China; [6] Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen, 518120, China; [7] Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, China

年份：2022

卷号：163

外文期刊名：LWT

收录：EI(收录号：20222112156938)、Scopus(收录号：2-s2.0-85130517043)

语种：英文

外文关键词：Metabolites - Bacteriology - Vitamins - Genes

外文摘要：Bacillus velezensis TPS3N, Bacillus subtilis TPS4, and Bacillus amyloliquefaciens TPS17 showing probiotic traits were isolated from the gut of Nile tilapia. However, the genetic information of these strains is obscure. Therefore, the current investigation was undertaken to prove the probiotic traits of B. velezensis TPS3N, B. subtilis TPS4, and B. amyloliquefaciens TPS17 through genomic analysis. Genome mining revealed gene clusters responsible for acid and bile (stress) tolerance as well as adhesion and colonization of the host's gut. Gene markers coding for a wide range of bacteriocins and secondary metabolites were also identified in the genome of the three isolates, suggesting that the three isolates could be used in place of traditional antibiotics. Genomic analysis also showed that the three isolates could boost the host's immunity and help digest complex feed ingredients by secreting numerous useful enzymes. Putative genes responsible for the synthesis of 6 vitamins and 11 amino acids (essential and non-essential) were found in the genome of the three isolates; coupled with their enzymatic capacity showed they could also be useful in other industrial applications. The current study provides a better understanding of the three isolates and provides an avenue for enhancing these strains through genetic manipulations. ? 2022 The Authors