文献类型：期刊文献

英文题名：Effects of dexamethasone on the morphology, gene expression and hepatic histology in adult female mosquitofish (Gambusia affinis)

作者：Zhong, Lishan[1];Liang, Yan-Qiu[1,2];Lu, Mixue[1];Pan, Chang-Gui[3];Dong, Zhongdian[4];Zhao, Hui[1,2];Li, Chengyong[1];Lin, Zhong[1];Yao, Li[5]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhanjian, Zhanjiang 524000, Peoples R China;[3]Guangxi Univ, Guangxi Lab Study Coral Reefs South China Sea, Nanning 530004, Peoples R China;[4]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China;[5]Guangdong Acad Sci, Guangdong Inst Anal, China Natl Analyt Ctr, Guangzhou 510070, Peoples R China

年份：2021

卷号：274

外文期刊名：CHEMOSPHERE

收录：SCI-EXPANDED(收录号：WOS:000642944800075)、、EI(收录号：20210609894534)、Scopus(收录号：2-s2.0-85100407034)、WOS

基金：The authors would like to acknowledge the financial support from the National Natural Science Foundation of China (41907346, 41907366 and 42076162), Natural Science Foundation of Guangdong Province (2019A1515011889, 2019A1515010382 and 2020A1515010496), Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) (ZJW-2019-08), Scientific Research Fund for South China Sea Scholars Program of Guangdong Ocean University (002029002011) and PhD Start Fund of Guangdong Ocean University (R17075).

语种：英文

外文关键词：Dexamethasone; Gambusia affinis; Masculinization; Gene expression; Hepatotoxicity

外文摘要：Glucocorticoids (GCs), including natural hormones as well as synthetic chemicals, can pose influences on physiological performance, development and reproduction of fish. Dexamethasone (DEX) is a synthetic glucocorticoid widely used as pharmaceutical and usually exists in effluents with varying degrees of concentrations. In this study, adult female mosquitofish (Gambusia affinis) were treated by DEX at concentrations of 0, 0.5, 5 and 50 mu g/L for 60 days. Morphological parameters of anal fin and skeleton, mRNA expression abundance, and histological alterations of liver were investigated to assess effects of DEX on mosquitofish. The results showed that DEX increased number of sections of ray 3 in anal fin and decreased 16L, 15D and 16D in skeletal parameters, which indicates DEX could potentially lead to weak masculinization. Furthermore, transcriptional expression levels of AR alpha, AR beta, ER beta, VTGC and CYP19A genes were notably down-regulated by DEX, which will contribute to weak masculinization in females. In addition, the damage to liver tissue was also induced by DEX. Taken together, this research demonstrated that aquatic environments contaminated by DEX have negative effects on mosquitofish at a population level. (C) 2021 Elsevier Ltd. All rights reserved.