文献类型：期刊文献

英文题名：Cloning, expression and functional characterization on vitellogenesis of estrogen receptors in Scatophagus argus

作者：Cui, Xue-Fan[1];Zhao, Yuan[1];Chen, Hua-Pu[1];Deng, Si-Ping[1];Jiang, Dong-Neng[1];Wu, Tian-Li[1];Zhu, Chun-Hua[1];Li, Guang-Li[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Key Lab Aquaculture South China Sea Aquat Econ An, Key Lab Marine Ecol & Aquaculture Environm Zhanji, Zhanjiang 524088, Peoples R China

年份：2017

卷号：246

起止页码：37

外文期刊名：GENERAL AND COMPARATIVE ENDOCRINOLOGY

收录：SCI-EXPANDED(收录号：WOS:000399860300005)、、Scopus(收录号：2-s2.0-85016164726)、WOS

基金：This study was supported by grants from Zhanjiang Science and Technology Bureau (2016A03017), Natural Science Foundation of Guangdong Province (2016A030313743), Guangdong Ocean University Natural Science Research Program (2015 and 2016), Project of Provincial Key Platform and Major Scientific Research of Colleges and Universities in Guangdong (2015KTSCX058), Sail Projects of Guangdong (2014.1), Marine Fishery Science and Technology Extension Projects of Guangdong (A201408A06, A201608801 and the 2017 project: Demonstration and popularization of the technology for desalination of breed in Spotted scat).

语种：英文

外文关键词：Scatophagus argus; Estrogen; Estrogen receptor; Estrogen receptor antagonist; Vitellogenin

外文摘要：Estrogen receptors (Er) play a critical role in vitellogenesis. Three ers (er alpha, er beta 1 and er beta 2) and vitellogenins (vtg-A, vtg-B and vtg-C) subtypes were isolated in various fish species, while the contribution of each Er to the regulation of vtgs expression was not analyzed in detail. Here, er alpha, er beta 1 and er beta 2 were cloned and all were found to be expressed in female liver in Scatophagus argus. During proteic vitellogenesis stage, er alpha was simultaneously up-regulated, while er beta 1 and er beta 2 were not, with three vtgs in female liver. The effects of 17 beta-estradiol (E-2) alone or combined with Er antagonists on ers, vtgs mRNA expressions and Vtg protein content in incubated male liver were examined by real-time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. The expressions of er alpha, er beta 1, vtgs mRNA and Vtg protein increased significantly after 24 h incubation with E-2 (0.1, 1 and 10 mu M), while Er nonselective antagonist ICI 182 780 (0.01, 0.1 and 1 mu M) significantly attenuated the up-regulation effects of E-2 on ers, vtgs mRNA and Vtg protein in a dose-dependent manner. Era selective antagonist Methyl-piperidinopyrazole (MPP) (0.01, 0.1 and 1 mu M) significantly attenuated the up-regulation effects of Ey on era, vtg-B, vtg-C mRNA and Vtg protein, while promoted the expression of er beta 1 and vtg-A. Er beta selective antagonist Cyclofenil (0.01, 0.1 and 1 mu M) attenuated the up-regulation effects of E-2 on ern er beta 2, vtg-A, vtg-C mRNA and Vtg protein while promoted the expression of er alpha and vtg-B. Our results suggest that the regulation of Ers on different vtgs was divergent in S. argus. (C) 2017 Elsevier Inc. All rights reserved.