文献类型：期刊文献

英文题名：A novel glyceroglycolipid from brown algae Ishige okamurae improve photoaging and counteract inflammation in UVB-induced HaCaT cells

作者：Xiao, Zhenbang[1];Yang, Shengtao[1];Liu, Yi[1];Zhou, Chunxia[1,2];Hong, Pengzhi[1,2];Sun, Shengli[1];Qian, Zhong-Ji[1,2]

机构：[1]Guangdong Ocean Univ, Shenzhen Inst Guangdong Ocean Univ, Coll Food Sci & Technol, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab, Zhanjiang 524025, Peoples R China

年份：2022

卷号：351

外文期刊名：CHEMICO-BIOLOGICAL INTERACTIONS

收录：SCI-EXPANDED(收录号：WOS:000721103300002)、、Scopus(收录号：2-s2.0-85118542364)、WOS

基金：The research was funded by the 2020 Shenzhen International Sci-entific and Technological Cooperation R&D Project (GJHZ20190823111601682) and Guangdong Basic and Applied Basic Research Foundation (2020A1515011075) . The supported by the Development Project about Marine Economy Demonstration of Zhan-jiang City (XM-202008-01B1) and Southern Marine Science and Engi-neering Guangdong Laboratory (Zhanjiang, ZJW-2019-07) .

语种：英文

外文关键词：Brown Algae; Ishige okamurae; Ishigoside; UVB; MMPs; AP-1

外文摘要：Background: Excessive exposure to Ultraviolet (UV) rays can cause premature skin aging. Ishigoside (IGS) is a new glyceroglycolipid compound isolated from brown algal Ishige okamurae, However, whether it can protect the skin from (Ultraviolet-B) UVB damage has not been illuminated. Methods: The in vitro anti-photoaging effect of IGS was conducted in UVB-induced HaCaT. The HaCaT cells were divided into the following five groups: (1) cells didn't suffer from UVB irradiation or IGS treatment. (2-5) Cells were treated with various concentrations of IGS (0, 10, 50, and 100 mu M) and irradiated by 40 mJ/cm2 UVB. The Matrix metalloproteinase (MMP) of photoaging process was determined by ELISA kits and the latent interaction between IGS and MMP was further performed by molecular docking. The crucial signaling pathway proteins involved in the collagen synthesis and degradation were subsequently evaluated by Western blotting, immunofluorescence and EMSA. Results: IGS effectively suppresses the high expressions and secretions of matrix metalloproteinases (MMPs) and photo-inflammation by blocking MAPKs, AP-1 and NF-kappa B. Meanwhile, increasing antioxidant enzyme expression. Molecular docking results suggest that inhibition of IGS on MMPs may be attributed to its hydrogen supply and hydrophobic capacity. In addition, IGS enhanced procollagen production by upregulating the TGF-beta/Smad pathways. Conclusions: IGS exhibited anti-photoaging activity in UVB-damage HaCaT. These effects might be a contribution by its suppression of MMPs expression via MAPKs, AP-1 and NF-kappa B pathway and have anti-oxidative and antiinflammatory effects. Therefore, IGS has the great potential to become skin-care products or functional foods for preventing skin photoaging.