文献类型：期刊文献

英文题名：A visual detection strategy for SARS-CoV-2 based on dual targets-triggering DNA walker

作者：Zhao, Liting[1];Li, Ciling[1];Kang, Xinhuang[1];Li, Yubin[1,2,3]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Res Ctr Coastal Environm Protect & Ecol Resilience, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Lab Climate Resources & Environm Continental Shelf, Dept Educ Guangdong Prov, Zhanjiang 524088, Guangdong, Peoples R China

年份：2023

卷号：379

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：SCI-EXPANDED(收录号：WOS:000972026700001)、、EI(收录号：20230113341866)、Scopus(收录号：2-s2.0-85145397638)、WOS

基金：This work was supported by the foundation and applied foundation research Joint -Youth Fund project of Guangdong Province, China (2019A1515110648) , the project of Enhancing School with Innovation of Guangdong Ocean University, China (2020KZDZX1108) , Zhanjiang Key Laboratory of Comprehensive Utilization of Chemical Research for Marine Resources (A14457) , Zhanjiang Key Laboratory of Marine Bio- materials Research (A15445) , Project of Enhancing School with Inno- vation of Guangdong Ocean University (GDOU2017052502) .

语种：英文

外文关键词：SARS-CoV-2; DNA Walker; Silver coated glass slide; Biosensor

外文摘要：SARS-CoV-2, a highly transmissible and mutagenic virus, made huge threats to global public health. The detection strategies, which are free from testing site requirements, and the reagents and instruments are portable, are vital for early screening and play a significant role in curbing the spread. This work proposed a silver-coated glass slide (SCGS)/DNA walker based on a dual targets-triggering mechanism, enzyme-catalyzed amplification, and smartphone data analysis, which build a portable visual detection strategy for the SARS-CoV-2 RNA -dependent RNA polymerase (RdRp) gene. By this method, the detection was reflected by the ultraviolet absor-bance changes and visible color changes to the naked eye which was analyzed by Red-Green-Blue (RGB) data analysis via smartphone within 30 min, simplifying the detection process and shortening the detection time. Meanwhile, the dual targets-triggering mechanism and dual signal amplification strategy ensured detection specificity and sensitivity. Further, the practicability was verified by the detection of the real sample which provided this method an application potential in SARS-CoV-2 rapid detection.