文献类型：期刊文献

英文题名：Identification, functional characterization, and estrogen regulation on gonadotropin-releasing hormone in the spotted scat, Scatophagus argus

作者：Chen, Hua-pu[1];Cui, Xue-fan[1];Wang, Yao-rong[1];Li, Zhi-yuan[1];Tian, Chang-xu[1];Jiang, Dong-neng[1];Zhu, Chun-Hua[1];Zhang, Yong[2,3];Li, Shui-sheng[2,3];Li, Guang-li[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Guangdong Res Ctr Reprod Control & Breeding Techn, Southern Marine Sci & Engn Guangdong Lab Zhanjian, Zhanjiang, Peoples R China;[2]Sun Yat Sen Univ, State Key Lab Biocontrol, Guangzhou, Peoples R China;[3]Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou, Peoples R China

年份：2020

卷号：46

期号：5

起止页码：1743

外文期刊名：FISH PHYSIOLOGY AND BIOCHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000538967200002)、、Scopus(收录号：2-s2.0-85086178919)、WOS

基金：This study was supported by grants from SouthernMarine Science and Engineering Guangdong Laboratory (Zhanjiang) (ZJW-2019-06), National Key R&D Program of China (2018YFD0901203), Special key projects of Guangdong science and Technology Department's science and technology innovation strategy (2018B030311050), National Natural Science Foundation of China (Nos. 41706174 and 31702326) and Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals, Guangdong Ocean University (No. KFKT2019ZD07).

语种：英文

外文关键词：Scatophagus argus; Gonadotropin-releasing hormone; 17 beta-Estradiol; Reproduction; Feedback regulation

外文摘要：Gonadotropin-releasing hormone (GnRH) is a key neuropeptide of the reproductive system. However, little is known about the role of GnRH in the spotted scat (Scatophagus argus). Here, three GnRH subtypes (cGnRH-II, sGnRH, and sbGnRH) were identified in the spotted scat. cGnRH-II and sGnRH were only expressed in the brains and gonads of both male and female fish, exhibiting a tissue-specific expression pattern, while sbGnRH was expressed at different transcription levels in all examined tissues. During ovarian maturation, hypothalamus-associated sbGnRH was upregulated, while the expression of sGnRH was variable and cGnRH-II first increased and then decreased. In vivo experiments showed that sbGnRH significantly promoted the expression of fsh and lh genes in a dose-dependent manner and exhibited a desensitization effect on lh expression at high concentrations. For sGnRH and cGnRH-II, only high concentrations could induce fsh and lh expression. Furthermore, treatment with highly concentrated sbGnRH peptide also induced fsh and lh expression, whereas the sGnRH and cGnRH-II peptides only induced fsh expression in vitro. 17 beta-Estradiol (E-2) significantly inhibited the expression of sbGnRH mRNA in a dose-dependent manner and did not impact sGnRH and cGnRH-II mRNA levels in vivo or in vitro. The inhibitory effect of E-2 on sbGnRH expression was attenuated by the estrogen receptor (ER) broad-spectrum antagonist (fulvestrant) and the ER alpha-specific antagonist (methyl-piperidinopyrazole), respectively, implying that the feedback regulation on sbGnRH is mediated via ER alpha. This study provides a theoretical basis for the reproductive endocrinology of the spotted scat by studying GnRH.