文献类型：期刊文献

英文题名：Effect of Carboxymethylation and Phosphorylation on the Properties of Polysaccharides from Sepia esculenta Ink: Antioxidation and Anticoagulation In Vitro

作者：Liu, Huazhong[1];Li, Fangping[1];Luo, Ping[1]

机构：[1]Guangdong Ocean Univ, Coll Chem & Environm, Zhanjiang 524088, Peoples R China

年份：2019

卷号：17

期号：11

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000502262200014)、、Scopus(收录号：2-s2.0-85074545769)、WOS

基金：This work was supported by the Natural Science Foundation of Guangdong Province, China (2016A030313753), and the Science and Technology Project on Special Fund for PublicWelfare Research and Ability Construction of Guangdong Province, China (2017A010105010).

语种：英文

外文关键词：Sepia esculenta ink polysaccharide; carboxymethylation; phosphorylation; antioxidation; anticoagulation

外文摘要：To investigate the effect of carboxymethylation and phosphorylation modification on Sepia esculenta ink polysaccharide (SIP) properties, this study prepared carboxymethyl SIP (CSIP) with the chloracetic acid method, and phosphorylated SIP (PSIP) with the sodium trimetaphosphate (STMP)/sodium tripolyphosphate (STPP) method, on the basis of an orthogonal experiment. The in vitro antioxidant and anticoagulant activities of the derivatives were determined by assessing the scavenging capacity of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals, which activated the partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). The results showed that SIP was modified successfully to be CSIP and PSIP, and degrees of substitution (DSs) of the two products were 0.9913 and 0.0828, respectively. Phosphorylation efficiently improved the antioxidant property of SIP, and the IC50 values of PSIP on DPPH and hydroxyl radicals decreased by 63.25% and 13.77%, respectively. But carboxymethylation reduced antioxidant activity of the native polysaccharide, IC50 values of CSIP on the DPPH and hydroxyl radicals increased by 16.74% and 6.89%, respectively. SIP significantly prolonged the APTT, PT, and TT in a dose-dependent fashion, suggesting that SIP played an anticoagulant action through intrinsic, extrinsic, and common coagulation pathways. CSIP and PSIP both possessed a stronger anticoagulant capacity than SIP via the same pathways; moreover, CSIP was observed to be more effective in prolonging APTT and PT than PSIP.