文献类型：期刊文献

中文题名：奶牛乳腺组织中ACSL5基因的表达量差异分析

英文题名：Analysis of the expression level of ACSL5 gene in mammary tissues of dairy cows

作者：王梦岩[1];姜平[1];余湘[1];葛言亮[1];孙豪[1];靳子康[2];刘娟[2];房希碧[1];杨润军[1];赵志辉[1,2]

机构：[1]吉林大学动物科学学院,吉林长春130062;[2]广东海洋大学农学院,广东湛江524088

年份：2019

卷号：39

期号：1

起止页码：100

中文期刊名：中国兽医学报

外文期刊名：Chinese Journal of Veterinary Science

收录：CSTPCD、、北大核心2017、CSCD_E2019_2020、北大核心、CSCD

基金：国家自然科学基金资助项目(31672389);农业部动物遗传育种与繁殖(家禽)重点实验室开放课题资助项目(poultrylab2018-4);吉林省中青年科技创新领军人才及团队资助项目(20170519014JH)

语种：中文

中文关键词：ACSL5;中国荷斯坦奶牛;qRT-PCR;乳蛋白

外文关键词：ACSL5;Holstein cows;qRT-PCR;milk protein

中文摘要：旨在分析高蛋白和低蛋白中国荷斯坦奶牛的乳腺组织中ACSL5基因表达量差异。以高蛋白的中国荷斯坦奶牛及低蛋白的中国荷斯坦奶牛为研究对象,分别提取乳腺组织总RNA,运用实时定量荧光PCR技术,检测奶牛乳腺组织中ACSL5基因的表达量。结果显示:在所检测的6头不同奶牛的乳腺组织中,ACSL5基因均有表达,且在高蛋白奶牛乳腺组织中的表达量显著低于低蛋白奶牛的乳腺组织(P<0.05)。结果表明,本试验初步验证ACSL5基因的表达可能与奶牛的乳蛋白具有相关关系,为进一步对ACSL5基因的生物学功能和作用机制的研究奠定了基础。

外文摘要：The purpose of the study was to analyze the differential expression of ACSL5 gene in mammary tissues of Chinese Holstein cows with high milk protein and low milk protein.The total RNA of mammary tissues was extracted from three high protein and three low protein of Chinese Holstein cows,respectively.The expression of ACSL5 gene in mammary tissues were detected by real-time quantitative fluorescence polymerase chain reaction(qRT-PCR).The results showed that ACSL5 gene was expressed in the mammary gland tissues of the six different cows tested,and the expression of ACSL5 gene in high milk protein cows were significantly lower than that in low milk protein cows(P<0.05).Therefore,ACSL5 gene expression may be significantly correlated with the milk protein rate of cows.Our study is the foundation for further study of the biological function and mechanism of ACSL5 gene.