文献类型：期刊文献

中文题名：洗脱剂极性对沉积物长链烯酮硅胶柱层析效果的影响

英文题名：Influence of Eluent Polarity on Separation of Sediment Long-Chain Alkenones in Silica Gel Column Chromatography

作者：陈国生[1];孔德明[1,2,3];邱梓惠[1];冯伟家[1];杨嘉雯[1]

机构：[1]广东海洋大学海洋与气象学院,广东湛江524088;[2]陆架及深远海气候、资源与环境广东省高等学校重点实验室,广东湛江524088;[3]广东海洋大学深圳研究院,广东深圳518108

年份：2022

卷号：42

期号：5

起止页码：82

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD、、北大核心、北大核心2020

基金：国家重点研发计划课题(2018YFA0605601);国家自然科学基金(41606070);广东海洋大学科研启动经费资助项目(R17011)。

语种：中文

中文关键词：长链烯酮;海洋沉积物;柱层析;洗脱剂;硅胶柱

外文关键词：alkenones;marine sediment;column chromatography;elution solvent;silica gel column

中文摘要：【目的】研究洗脱溶剂极性对海洋沉积物中C_(37)和C_(38)烯酮的柱层析分离效果及对重建古海表温度的影响,探寻能完全洗脱长链烯酮而不引入过多杂质的洗脱溶剂配比。【方法】通过改变正己烷(H)与二氯甲烷(D)体积比来调节洗脱液极性,按极性从小到大对标准样品进行洗脱,并计算每种烯酮分子的洗脱量。【结果】当V_(H)∶V_(D)=8∶1时烯酮开始被洗脱出,到1∶2时可被完全洗脱。烯酮C_(38:2)Et最先被洗脱出,而C_(37:3)最后被洗脱出。各烯酮分子被洗脱量的峰值出现在不同极性的洗脱液,按极性排序为C_(38:2)Et

外文摘要：【Objective】This study aims to explore the influence of elution solvent polarity on the separation of C_(37)and C_(38)alkenones in marine sediments by column chromatography and its effect on reconstructed sea surface temperature,to find the solvent ratio that can completely elute all C_(37)and C_(38)alkenones without introducing too much impurities.【Method】In this study,the polarity of the eluent is adjusted by changing the ratio of n-hexane(H)and dichloromethane(D).The standard samples are eluted with increasing polarity,and calculate the eluted amount of each alkenone.【Result】Alkenones start to be eluted when V_(H)∶V_(D)=8∶1,and can be completely eluted when V_(H)∶V_(D)=1∶2.C_(38:2)Et is the first to be eluted,and C_(37:3)is the last.The maximum percentage of each eluted alkenones appears in the eluent with different polarities,and the order of the eluent polarity is C_(38:2)Et