文献类型：期刊文献

英文题名：Algiphilus acroporae sp. nov. and Coraliihabitans acroporae gen. nov. sp. nov., isolated from scleractinian coral Acropora digitifera

作者：Sun, Hao[1,2,3];Zheng, Huina[4];Liao, Baolin[1];Chen, Bogui[1];Li, Aihua[3];Xiao, Baohua[1,2]

机构：[1]Guangdong Ocean Univ, Shenzhen Inst, Binhai 2 Rd, Shenzhen 518120, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[3]Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China;[4]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China

年份：2022

卷号：72

期号：4

外文期刊名：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000800144400002)、、WOS

基金：This work was financially supported by projects from Shenzhen Science and Technology R&D Fund (KJYY20180213182720347 and JCYJ20200109144803833) and Guangdong Key Area R&D Program Project (2020B1111030002).

语种：英文

外文关键词：Algiphilus acroporae sp. nov. Coraliihabitans acroporae gen. nov. sp. nov; Acropora digitifera; scleractinian coral

外文摘要：Two Gram-staining-negative, aerobic, rod-shaped bacteria NNCM1(T) and NNCM2(T) were isolated from the scleractinian coral Acropora digitifera. NNCM1(T) grew with 0.5-12% (w/v) NaCl (optimum, 3-6%), at 18-37 degrees C (optimum, 28 degrees C) and at pH 6.0-10.0 (optimum, 7.0-8.0). NNCM2(T) grew with 0.5-10% (w/v) NaCl (optimum, 2 %), at 18-37 degrees C (optimum, 28 degrees C) and at pH 6.5-9.0 (optimum, 7.0). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that NNCM1(T) formed a lineage within the genus Algiphilus of the family Algiphilaceae, and it was distinct from the most closely related species Algiphilus aromaticivorans DG1253(T), with a 16S rRNA gene sequences similarity of 97.05%. NNCM2(T) formed a lineage within the family Rhodobacteraceae, and it was distinct from the closely related genera Limibaculum halophilum CAU 1123(T), Paroceanicella profunda D4M1(T) and Pseudoruegeria aestuarii MME-001(T) with 93.41, 92.78 and 91.09% identities, respectively. The major respiratory quinone was Q-8 and Q-10 for NNCM1(T) and NNCM2(T), respectively. The predominant fatty acids (more than 10%) were summed feature 8 (39.4%) and C-16(:0) (19.4%) for NNCM1(T) and summed feature 8 (62.8%) and C-1(6:)0 (12.4%) for NNCM2(T). The DNA G+C contents of NNCM1(T) and NNCM2(T) were 63.3 and 63.4 mol% respectively. The polar lipids of NNCM1(T) comprised one diphosphatidylglycerol, one phosphatidylethanolamine, one phosphatidylglycerol and one unknown polar lipid, while those of NNCM2(T) comprised one phosphatidylethanolamine, one phosphatidylglycerol, one aminolipid and four unknown polar lipids. Phenotypic characteristics (physiological, biochemical and chemotaxonomic) also supported the taxonomic novelty of the two isolates. Thus, NNCM1(T) is considered to represent a novel species within genus Algiphilus, for which the name Algiphilus acroporae sp. nov. is proposed. The type strain is NNCM1(T) (=KCTC 82966(T)=MCCC 1K06445(T)). NNCM2(T) represents a novel genus and species within the family Rhodobacteraceae, for which the name Coraliihabitans acroporae gen. nov. sp. nov. is proposed. The type strain is NNCM2(T )(=KCTC 82967(T)=MCCC 1K06408(T)).