文献类型：期刊文献

英文题名：Genome Survey of Male and Female Spotted Scat (Scatophagus argus)

作者：Huang, Yuanqing[1];Jiang, Dongneng[1];Li, Ming[1];Mustapha, Umar Farouk[1];Tian, Changxu[1];Chen, Huapu[1];Huang, Yang[1];Deng, Siping[1];Wu, Tianli[1];Zhu, Chunhua[1];Li, Guangli[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Guangdong Res Ctr Reprod Control & Breeding Techn, Zhanjiang 524088, Peoples R China

年份：2019

卷号：9

期号：12

外文期刊名：ANIMALS

收录：SCI-EXPANDED(收录号：WOS:000506636400115)、、WOS

基金：This study was supported by grants from the Key Project of "Blue Granary Science and Technology Innovation" of the Ministry of Science and Technology (2018YFD0901203); the National Natural Science Foundation of China (Nos. 31702326 and 41706174); Natural Science Foundation of Guangdong Province (2018B030311050); Guangdong Basic and Applied Basic Research Foundation (2019A1515012042 and 2019A1515010958); Independent Project of Guangdong Province Laboratory (ZJW-2019-06); grant from the Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams (2019KJ149); Department of Education of Guangdong Province (2018KTSCX090); Program for Scientific Research Start-Up Funds of Guangdong Ocean University.

语种：英文

外文关键词：genome size; illumina sequencing; Dmrt1; sex determining gene; aquaculture; sex control

外文摘要：Simple Summary The spotted scat, Scatophagus argus, is a marine aquaculture fish species that is economically important in Asia. As the spotted scat exhibits notable sexual dimorphism with respect to growth, aquaculture efficiency can be increased through the practice of sex control breeding. However, genomic data from S. argus is lacking. In the present study, a genomic survey was conducted using next-generation sequencing technologies. Data, including the size of the genome, sequence repeat ratio, heterozygosity ratio, whole genome sequence and gene annotation were obtained. This information will serve to support the breeding and aquaculture of S. argus. Abstract The spotted scat, Scatophagus argus, is a species of fish that is widely propagated within the Chinese aquaculture industry and therefore has significant economic value. Despite this, studies of its genome are severely lacking. In the present study, a genomic survey of S. argus was conducted using next-generation sequencing (NGS). In total, 55.699 GB (female) and 51.047 GB (male) of high-quality sequence data were obtained. Genome sizes were estimated to be 598.73 (female) and 597.60 (male) Mbp. The sequence repeat ratios were calculated to be 27.06% (female) and 26.99% (male). Heterozygosity ratios were 0.37% for females and 0.38% for males. Reads were assembled into 444,961 (female) and 453,459 (male) contigs with N50 lengths of 5,747 and 5,745 bp for females and males, respectively. The average guanine-cytosine (GC) content of the female genome was 41.78%, and 41.82% for the male. A total of 42,869 (female) and 43,283 (male) genes were annotated to the non-redundant (NR) and SwissProt databases. The female and male genomes contained 66.6% and 67.8% BUSCO core genes, respectively. Dinucleotide repeats were the dominant form of simple sequence repeats (SSR) observed in females (68.69%) and males (68.56%). Additionally, gene fragments of Dmrt1 were only observed in the male genome. This is the first report of a genome-wide characterization of S. argus.