文献类型：期刊文献

英文题名：Identification of a tyrosinase gene and its functional analysis in melanin synthesis of Pteria penguin

作者：Yu, Feifei[1];Pan, Zhenni[1];Qu, Bingliang[1];Yu, Xiangyong[2];Xu, Kaihang[1];Deng, Yuewen[1];Liang, Feilong[1]

机构：[1]Guangdong Ocean Univ, Fishery Coll, 40 East Jiefang Rd, Xiashan Dist 524025, Zhanjiang, Peoples R China;[2]South China Agr Univ, Ocean Coll, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China

年份：2018

卷号：656

起止页码：1

外文期刊名：GENE

收录：SCI-EXPANDED(收录号：WOS:000430904000001)、、WOS

基金：This work was supported by the Guangdong Provincial Science and Technology Program (2016A020210115); Guangdong Marine Fishery Development Foundation (B201601-Z08); Guangdong Major Project of Innovation School (GDOU2016050248); Outstanding Young Teacher Foundation of Guangdong Ocean University (2014004) and Doctoral Scientific Research Foundation of Guangdong Ocean University (E15041).

语种：英文

外文关键词：Tyrosinase; Pteria penguin; Melanin; RNA interference; LC-MS/MS

外文摘要：Tyrosinase is a key rate-limiting enzyme in melanin synthesis. In this study, a new tyrosinase gene (Tyr) was identified from Pteria penguin and its effect on melanin synthesis was deliberated by RNA interference (RNAi). The cDNA of PpTyr was 1728 bp long, containing a 5'untranslated region (UTR) of 11 bp, a 3'UTR of 295 bp, and an open reading fragment of 1422 bp encoding 473 amino acids. Amino acid alignment showed PpTyr had the highest (50%) identity to tyrosinase-like protein 1 from Pinctada fucata. Phylogenetic tree analysis classified PpTyr into alpha-subclass of type-3 copper protein. Tissue expression analysis indicated that PpTyr was highly expressed in mantle, a nacre formation related tissue. After PpTyr RNA interference, PpTyr mRNA was significantly inhibited by 71.0% (P < 0.05). For other melanin-related genes, PpCreb2 and PpPax3 expression showed no significant change, but PpBcl2 was obviously increased. By liquid chromatograph-tandem mass spectrometer (LC-MS/MS) analysis, the total content of PDCA (pyrrole-2, 3-dicarboxylic acid) and PTCA (pyrrole-2,3,5-tricarboxylic acid), as main markers for eumelanin, was sharply decreased by 66.6% after PpTyr RNAi (P < 0.05). The percentage of PDCA was also obviously decreased from 20.1% to 13.9%. This indicated that tyrosinase played a key role in melanin synthesis and color formation of P. penguin.