文献类型：期刊文献

英文题名：Bactericidal efficacy of plasma-activated water against Vibrio parahaemolyticus on Litopenaeus vannamei

作者：Zhang, Huanlan[1];Wei, Jie[1];Xv, Hongjie[1];Khan, Imran[2];Sun, Qinxiu[1];Zhao, Xihong[3];Gao, Jialong[1];Liu, Shucheng[1,4];Wei, Shuai[1,4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol,Key Lab Adv Proc Aquat Pro, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Guangdong Prov Engn Technol Res Ctr Seafood,Guangd, Zhanjiang 524088, Peoples R China;[2]Univ Haripur, Dept Food Sci & Technol, Haripur, Pakistan;[3]Wuhan Inst Technol, Sch Environm Ecol & Biol Engn, Wuhan, Peoples R China;[4]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian, Peoples R China

年份：2024

卷号：11

外文期刊名：FRONTIERS IN NUTRITION

收录：SCI-EXPANDED(收录号：WOS:001187951800001)、、Scopus(收录号：2-s2.0-85188159681)、WOS

基金：The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by the National Key R&D Program of China (2023YFD2401502), Key Project of Department of Education of Guangdong Province (2022ZDZX4014), China Agriculture Research System (CARS-48), and Innovation Team Program of Guangdong Ocean University (CXTD2024004).

语种：英文

外文关键词：plasma-activated water; Vibrio parahaemolyticus; inactivation; Litopenaeus vannamei; cell membrane

外文摘要：In this study, the antimicrobial mechanism of plasma-activated water (PAW) against Vibrio parahaemolyticus and the effectiveness of PAW in artificially contaminated Litopenaeus vannamei were investigated. The results demonstrated a significant reduction (p < 0.05) in viable counts of V. parahaemolyticus with increasing plasma discharge time (5, 10, 20, and 30 min) and PAW immersion time (3, 5, 10, 20, and 30 s). Specifically, the count of V. parahaemolyticus decreased by 2.1, 2.7, 3.3, and 4.4 log CFU/mL after exposed to PAW 5, PAW 10, PAW 20, and PAW 30 for 30 s, respectively. Significant cell surface wrinkling, accompanied by notable nucleic acid and protein leakage were observed after treatment with PAW. The permeability of the inner and outer cell membranes was significantly increased (p < 0.05), along with an increase in electrical conductivity (p < 0.05). The reactive oxygen species (ROS) within V. parahaemolyticus cells were significantly increased (p < 0.05), while superoxide dismutase (SOD) activity, and the relative expression of the ompW, emrD, and luxS genes were significantly decreased (p < 0.05). A reduction number of 1.3, 1.8, 2.1, and 2.2 log CFU/g of V. parahaemolyticus in artificially contaminated L. vannamei was obtained with PAW for 5 min. The study elucidated that PAW could destroy cell membranes, leading to cell death. The findings would strengthen strategies for V. parahaemolyticus control and provide a potential application of PAW for preserving aquatic products.