文献类型：期刊文献

中文题名：火龙果溃疡病病原菌鉴定及生物学特性

英文题名：Identification and biological characteristics of Neoscytalidium dimidiatum causing pitaya canker

作者：易润华[1];甘罗军[1];晏冬华[1];吴泽菁[1];童依婷[1];吴凤发[1]

机构：[1]广东海洋大学农学院生物技术系

年份：2013

卷号：40

期号：2

起止页码：102

中文期刊名：植物保护学报

外文期刊名：Acta Phytophylacica Sinica

收录：CSTPCD、、北大核心2011、北大核心、CSCD、CSCD2013_2014

基金：广东省科技厅农业科技攻关项目(2008B020500007)

语种：中文

中文关键词：火龙果;溃疡病;新暗色柱节孢;生物学特性

外文关键词：pitaya ; canker disease ; Neoscytalidium dimidiatum ; biological characteristic

中文摘要：为确定火龙果溃疡病病原菌,应用柯赫氏法则测定了从病样中分离的纯培养物的致病性,根据形态学特性和ITS序列分析对病原菌进行鉴定,并采用菌落生长法和玻片法研究了其生物学特性。结果表明,火龙果溃疡病的病原菌为新暗色柱节孢Neoscytalidium dimidiatum(Penz.)Crous&Slippers。碳氮源对病原菌菌丝生长和产孢量的影响差异明显,菌丝生长和产孢的最佳碳源为蔗糖,菌丝生长最佳氮源为酵母膏,产孢最佳氮源为甘氨酸。温度、pH对病原菌菌丝生长、产孢和孢子萌发的影响差异显著,菌丝生长、产孢、孢子萌发的最佳温度均为30℃;菌丝生长最佳pH为8,产孢和孢子萌发最佳pH为7。光照对病原菌菌丝生长和产孢有抑制作用,对孢子萌发没有明显影响。相对湿度高有利于孢子萌发,高于98%时,萌发率达100%。

外文摘要：In order to confirm the pathogen of pitaya canker, the pathogenicity of pure culture isolated from the samples of disease tissues was tested according to Koch＇ s law; the pathogen was identified ac- cording to morphological characteristics and ITS sequence analysis, biological characteristics were deter- mined using colony growth and slide methods. The results showed pitaya canker was caused by Neoscytal- idium dimidiatum （Penz.） Crous ＆ Slippers. Effects of carbon and nitrogen sources on mycelial growth and sporulation were significant, optimal carbon source for mycelial growth and sporulation was sucrose, optimal nitrogen source for mycelial growth was yeast extract and for sporulation was glycine. Effects of temperature and pH on mycelial growth, sporulation and spore germination had significant differences, optimal temperature for mycelial growth, sporulation and spore germination was 30 ℃ ; optimal pH value for sporulation and spore germination was 7.0 and pH 8.0 for mycelial growth. Light could inhibit on my- celial growth and sporulation, but not on spore germination. High relative humidity （RH） was advanta- geous to spore germination, when RH was higher than 98% , the percentage of spore germination was up to 100%.