详细信息
Toxicologic effect of short-term enrofloxacin exposure on brain of Carassius auratus var. Pengze ( SCI-EXPANDED收录 EI收录) 被引量:8
文献类型:期刊文献
英文题名:Toxicologic effect of short-term enrofloxacin exposure on brain of Carassius auratus var. Pengze
作者:Lin, Zhen[1];Wan, Gen[2];Wu, Jiayi[1];Liu, Huazhong[1,4];Zhang, Fan[2];Tang, Xiaochen[3];Ruan, Jiming[2]
机构:[1]Guangdong Ocean Univ, Coll Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang 330045, Peoples R China;[3]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[4]Guangdong Ocean Univ, Coll Chem & Environm Sci, 1 Haida Rd, Zhanjiang 524088, Guangdong, Peoples R China
年份:2023
卷号:869
外文期刊名:SCIENCE OF THE TOTAL ENVIRONMENT
收录:SCI-EXPANDED(收录号:WOS:000925380000001)、、EI(收录号:20230613541109)、Scopus(收录号:2-s2.0-85147197967)、WOS
基金:The study was supported by the National Natural Science Foundation of China (No. 31860735) , Guangdong Basic and Applied Basic Research Foundation (No. 2022A1515012091) and Special Project in Key Fields of Guangdong Universities (No. 2022ZDZX2025) .
语种:英文
外文关键词:Enrofloxacin; Blood-brain barrier; Transcriptome analysis; Brain damage; Crucian carp
外文摘要:To further explore short-term exposure of enrofloxacin (ENR) induced toxicity in crucian carp brain that has been reported by our previous work, as well as the possible toxicological mechanisms, this study investigated the blood-brain barrier (BBB) permeability to low dosage of ENR through comprehensively assessing expression of BBB constitutive molecules zonula occludens-1 (ZO-1) and permeability glycoprotein (P-gp), as well as ENR residue in brain of crucian carp. Toxicologic effect of ENR on brain tissue was determined through evaluating expression of brain-derived proteins S100B, neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP) in crucian carp brain tissue, as well as contents of the proteins in serum. The toxicological mechanisms were explored through analyzing transcriptome analysis data. Results showed that ENR possessed excellent permeability to crucian carp BBB, which was closely related to deranged BBB structure and declined ENR efflux that were attributed to downregulated expression of ZO-1 and P-gp by ENR exposure. Meanwhile, S100B, NSE and GFAP were upregulated in brain by ENR, and came out into blood across the damaged BBB. These data revealed that ENR induced disruption of BBB and damage of brain tissue in crucian carp. Transcriptome analysis data indicated that ENR induced toxicologic effect might be related to modification of metabolism, organismal systems, and genetic information processing, etc., and that PI3K/Akt, MAPK, HIF-1, and ubiquitin mediated proteolysis involved the mechanisms, most of the mechanisms were attributed to ENR induced oxidative stress in crucian carp brain.
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