文献类型：期刊文献

英文题名：Expression, purification and antibody preparation of flagellin FlaA from Vibrio alginolyticus strain HY9901

作者：Liang, H.Y.[1,2,3,4,5]; Xia, L.Q.[3]; Wu, Z.H.[3,4,5]; Jian, J.C.[3,4,5]; Lu, Y.S.[3,4,5]

机构：[1] South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; [2] Graduate School of Chinese Academy of Sciences, Beijing, China; [3] College of Fisheries, Guangdong Ocean University, Jiefang East Road, No. 40, Zhanjiang City, Guangdong Province 524025, China; [4] Guangdong Prov. Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, China; [5] Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China

年份：2010

卷号：50

期号：2

起止页码：181

外文期刊名：Letters in Applied Microbiology

收录：EI(收录号：20231113726873)、Scopus(收录号：2-s2.0-74049101584)

语种：英文

外文关键词：Affinity chromatography - Amino acids - Antibodies - Chromatographic analysis - Cloning - Electron microscopy - Escherichia coli - Organic acids

外文摘要：Aims: The main aims of this study were to clone and express flagellin flaA gene from Vibrio alginolyticus strain HY9901, also to prepare mouse anti-FlaA polyclonal antibody for future pathogen or vaccine study. Methods and Results: The full-length flaA gene was amplified by PCR with designed primers. The open reading frame of flaA gene contains 1131 bp, and its putative protein consists of 376 amino acid residues. Alignment analysis indicated that the FlaA protein was highly conserved. SDS-PAGE indicated that the FlaA protein was successfully expressed in Escherichia coli BL21 (DE3). Then, the recombinant FlaA protein was purified by affinity chromatography, and the mouse anti-FlaA serum was produced. The expression of flaA gene was verified by various immunological methods, including western blotting, enzyme-linked immunosorbent assay (ELISA) and immunogold electron microscopy (IEM). Conclusions: Flagellin flaA gene was cloned and identified from V. alginolyticus HY9901, the recombinant FlaA protein was expressed and purified, and high-titre FlaA protein-specific antibody was produced. Western blot analysis revealed that the prepared antiserum not only specifically react to FlaA fusion protein, but also to natural FlaA protein of V. alginolyticus. The expressed FlaA protein was demonstrated, for the first time, as the component of flagella from V. alginolyticus by IEM. Significance and Impact of the Study: This study may offer important insights into the pathogenesis of V. alginolyticus, provide a base for further studies on the diagnosis and evaluation that whether the FlaA protein could be used as an effective vaccine candidate against infection by V. alginolyticus and other Vibrio species. Additionally, the purified FlaA protein and polyclonal antibody can be used for further functional and structural studies. ? 2009 The Society for Applied Microbiology.