文献类型：期刊文献

英文题名：Activation of the endogenous acid protease in the head of Litopenaeus vannamei induced by UV-C irradiation

作者：Dou, Bijing[1];Wang, He[1];Cao, Wenhong[1,2,3,4,5];Chen, Zhongqin[1,2,3,4];Zhu, Guoping[1,2,3,4];Zhang, Chaohua[1,2,3,4,5];Zheng, Huina[1,2,3,4,5];Lin, Haisheng[1,2,3,4,5];Gao, Jialong[1,2,3,4,5]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Natl Res & Dev Branch Ctr Shellfish Proc Zhanjiang, Zhanjiang 524088, Peoples R China;[3]Guangdong Prov Engn Technol Res Ctr Seafood, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[4]Guangdong Higher Educ Inst, Guangdong Prov Engn Lab Marine Biol Prod, Key Lab Adv Proc Aquat Prod, Zhanjiang 524088, Peoples R China;[5]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China

年份：2023

卷号：58

期号：3

起止页码：1401

外文期刊名：INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000913061300001)、、WOS

基金：Acknowledgments This work was supported by the National Natural Science Foundation of China (Grant No. 32172163 and No. 41576151) and the Science and Technology Plan of Guangdong Province (2015A020209164). We are grateful for the comments and constructive suggestions provided by the anonymous referees to improve the manuscript.

语种：英文

外文关键词：Acid protease; activation; conformational analysis; Litopenaeus vannamei; UV-C irradiation

外文摘要：Previous studies have found that UV-C irradiation activated endogenous acid protease (EAP) in shrimp heads. Aiming at understanding the effect of UV-C irradiation on the activation of EAP and improving the utilisation of EAP, the effect of UV-C irradiation on the enzymatic properties and conformation of EAP was investigated. The enzymatic properties of EAP were unaltered after UV-C irradiation, and the optimum pH, temperature, and NaCl concentration were 3, 60 degrees C, and 0.5 mol/L, respectively. Ca2+ and Mg2+ activated the enzyme. The molecular weight of the purified endogenous acid protease (P-EAP) was 32 kDa. UV-Vis, FTIR, circular dichroism, and fluorescence spectroscopy indicated that the increase in enzyme activity by UV-C irradiation was related to an increase in tryptophan and hydrogen bonding, implying that it may be the change in the spatial structure of the protein that caused the activation of the enzyme. Results revealed that UV-C irradiation can be used to activate proteases and provide a reference for enhancing the enzymatic activity of other enzymes from shrimp heads.