文献类型：期刊文献

英文题名：Reproductive effects of synthetic progestin norgestrel in zebrafish (Danio rerio)

作者：Liang, Yan-Qiu[1];Huang, Guo-Yong[2];Lin, Zhong[1];Li, Jin[1];Yang, Jie-Wen[1];Zhong, Lai-Yuan[1];Ying, Guang-Guo[2,3]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Chinese Acad Sci, Guangzhou Inst Geochem, CAS Res Ctr PRD Environm Pollut & Control, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China;[3]South China Normal Univ, MOE Key Lab Environm Theoret Chem, Environm Res Inst, Guangzhou 510006, Guangdong, Peoples R China

年份：2018

卷号：190

起止页码：17

外文期刊名：CHEMOSPHERE

收录：SCI-EXPANDED(收录号：WOS:000414881600003)、、WOS

基金：The authors would like to acknowledge the financial support from the Natural Science Foundation of Guangdong Province (2017A030310662 and 2016A030310330), and the Department of Finance of Guangdong Province (002026002008). Thanks also to the two anonymous reviewers for their useful comments and suggestions.

语种：英文

外文关键词：Progestin; Norgestrel; Reproduction; Endocrine disruption; Gene transcription

外文摘要：The aim of this study was to assess the adverse effects of synthetic progestin norgestrel (NGT) on the reproduction of zebrafish by measuring the egg production, histology and transcriptional expression profiles along the hypothalamic-pituitary-gonadal (HPG) axis in adult zebrafish. After a pre-exposure period of 7 days, adult zebrafish were exposed to 6, 29 and 69 ng L-1 NGT for 21 days. The results showed that exposure to 69 ng L-1 NGT led to a significant up-regulation of follicle stimulating hormone, beta polypeptide (fshb), luteinizing hormone, beta polypeptide (lhb), progesterone receptor (pgr), estrogen receptor 1 (esr1) and androgen receptor (ar) genes in the brains, as well as significant up regulation of hydroxysteroid 20-beta dehydrogenase (hsd20b) and hydroxysteroid 11-beta dehydrogenase 2 (hsd11b2) genes and down-regulation of 11-beta-hydroxylase (cyp11b) gene in the ovaries of females. In the testes of males, an overall down-regulation of steroidogenic acute regulatory protein (star), cytochrome P450-mediated side-chain cleavage enzyme (cyp11a1), cyp11b, hsd20b, hydroxysteroid 17-beta dehydrogenase type 3 (hsd17b3), hsd11b2 and ar genes were observed following exposure to different treatments of NGT. These transcriptional alterations imply that NGT could exhibit the potent progestogenic and androgenic activities in zebrafish. Egg production as well as histology in the ovaries and testes was not affected by NGT. Taken together, the overall results demonstrated that NGT could significantly affect transcriptional expression levels of genes related to HPG axis in zebrafish, and whether that change translates to additional physiological effects is needed further research. (C) 2017 Published by Elsevier Ltd.