文献类型：期刊文献

中文题名：禁食暂养对卵形鲳鲹有水保活生理响应的影响

英文题名：Effects of Fasting Acclimation on the Physiological Response to Water Preservation of Trachinotus ovatus

作者：张坤[2];周结倩[1,2];范秀萍[1,2,3];魏帅[1,2,3];孙钦秀[1,2,3];夏秋瑜[1,2,3];刘书成[1,2,3]

机构：[1]南方海洋科学与工程广东省实验室(湛江),广东湛江524025;[2]广东海洋大学食品科技学院/广东省水产品加工与安全重点实验室/广东省海洋食品工程技术研发中心/广东省海洋生物制品工程重点实验室/水产品深加工广东普通高校重点实验室,广东湛江524088;[3]海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁大连116034

年份：2022

卷号：42

期号：1

起止页码：44

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD、、北大核心、北大核心2020

基金：南方海洋科学与工程广东省实验室(湛江)资助(ZJW-2019-06);广东普通高等学校海洋食品绿色加工技术研究团队(2019KCXTD011)。

语种：中文

中文关键词：禁食暂养;卵形鲳鲹;应激;有水保活;生理响应

外文关键词：fasting acclimation;Trachinotus ovatus;stress;keeping alive with water;physiological response

中文摘要：【目的】探究禁食暂养对卵形鲳鲹有水保活生理响应的影响,为卵形鲳鲹的保活运输奠定基础。【方法】通过测定暂养过程中水体的总氨氮和pH值、卵形鲳鲹血液生理生化指标、肌肉氧化应激指标等的变化,分析确定卵形鲳鲹有水保活的禁食暂养条件。【结果与结论】禁食暂养过程中,随着暂养时间的延长,水体总氨氮含量与pH值显著上升,但在禁食暂养时间为6 h时,其总氨氮质量浓度为3.22 mg/L和pH值为7.80;禁食暂养6 h可以降低或减少卵形鲳鲹的应激,使血液中乳酸脱氢酶(LDH)和谷草转氨酶(AST)活性以及皮质醇(Cortisol)、血糖(GLU)、尿素氮(BUN)和总胆固醇(T-CHO)含量处于较低水平,分别为2785.53 U/L、19.04 U/L、158.72 ng/mL、2.98 mmol/L、4.35 mmol/L和4.96 mmol/L;同时减轻了应激带来的肌肉组织氧化损失,使过氧化氢酶(CAT)活性和丙二醛(MDA)含量维持在较低水平,分别为6.34 U/L和5.86 mmol/L;卵形鲳鲹适宜的暂养条件为在鱼水比m鱼∶m水=1∶20和(25±1)℃的洁净海水中暂养6 h。

外文摘要：【Objectives】To explore the effect of fasting acclimation on the physiological response to water preservation of golden pompano,Trachinotus ovatus in order to lay down the foundation for the preservation and transportation of golden pompano【.Methods】By measuring the changes of total ammonia nitrogen and pH value of the water body,the blood physiological and biochemical indexes of golden pompano,and the oxidative stress index of muscle during the fasting acclimation process.The fasting acclimation conditions of golden pompano were analyzed and determined.【Results and Conclusions】The results showed that during the fasting acclimation,with the extension of fasting acclimation time,the total ammonia nitrogen content and pH value of the water body increased significantly.When the fasting acclimation time was 6 h,the total ammonia nitrogen content was 3.22 mg/L and the pH value was 7.80,the fasting acclimation for 6 hours can reduce or reduce the stress of the golden pompano,make the blood lactate dehydrogenase(LDH)and aspartate aminotransferase(AST)activity and the content of cortisol,blood sugar(GLU),blood urine nitrogen(BUN)and total cholesterol(T-CHO)at a lower level,Its activity and content are 2785.53 U/L,19.04 U/L,158.72 ng/mL,2.98 mmol/L,4.35 mmol/L and 4.96 mmol/L,respectively;at the same time,it reduces the oxidative loss of muscle tissue caused by stress,so that catalase(CAT)activity and malondialdehyde(MDA)content are maintained at a low level,Its activity and content are 6.34 U/L and 5.86 mmol/L;the suitable fasting acclimation conditions for golden pompano are the fish-water ratio of mfish to m water 1∶20 and(25±1)℃of clean seawater for 6 hours.