文献类型：期刊文献

英文题名：Comparative Study of Cytotoxicity, DNA Damage and Oxidative Stress Induced by Heavy Metals Cd(II), Hg(II) and Cr(III) in Yeast

作者：Wang, Jingwen[1];Fang, Zhijia[1];Gao, Jian[1];Sun, Lijun[1];Wang, Yaling[1];Liu, Ying[1];Gooneratne, Ravi[2]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol,Cunjin Coll,Key Lab Adv P, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Guangdong Prov Engn Technol,Res Ctr Marine Food, Zhanjiang 524088, Peoples R China;[2]Lincoln Univ, Dept Wine Food & Mol Biosci, Canterbury 7647, New Zealand

年份：2021

卷号：78

期号：5

起止页码：1856

外文期刊名：CURRENT MICROBIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000633269800001)、、Scopus(收录号：2-s2.0-85103134125)、WOS

基金：This work was supported by the National Natural Science Foundation of China (No. 31701706), the Program for Scientific Research Start-up Funds of the Guangdong Ocean University (No. R17102), Characteristic Innovation Project of Guangdong Province (No. 2018KTSCX089) and Guangdong Basic and Applied Basic Research Foundation (Nos. 2019A1515010809 and 2021A1515012443). The design of the study: the National Natural Science Foundation of China (No. 31701706); data collection, interpretation of data: the National Natural Science Foundation of China (No. 31701706), the Program for Scientific Research Start-up Funds of the Guangdong Ocean University (No. R17102), Characteristic Innovation Project of Guangdong Province (No. 2018KTSCX089), Guangdong Basic and Applied Basic Research Foundation (Nos. 2019A1515010809 and 2021A1515012443); writing the manuscript: the Program for Scientific Research Start-up Funds of the Guangdong Ocean University (No. R17102).

语种：英文

外文摘要：Wide range of applications of heavy metals and improperly discarded their castoffs possess serious threats to environment and human health. In this study, cytotoxicity, DNA damage and oxidative stress induced by Cd(II), Hg(II) and Cr(III) were comparatively studied in yeast Saccharomyces cerevisiae. Cd(II), Hg(II), and Cr(III) all produced strong cytotoxicity resulting in growth inhibition and cell mortality to varying degrees (Hg(II) > Cd(II) > Cr(III)). Hg(II) produced more oxidative stress. Cr(III) caused more serious DNA damage in vitro. Cd(II) also caused both obvious DNA damage and oxidative stress at higher concentration, but not as efficiently as Cd(II) and Hg(II). A further null mutation sensitivity assay showed that the relative sensitivity of rad1 increment to the metals was Cr(III) > Cd(II) > Hg(II), and that of trx1 increment to the metals was Hg(II) > Cd(II) > Cr(III). These data provide a clear evidence that the Cr(III) can cause significant DNA damage and potential genotoxicity; Hg(II) can strongly inhibit SOD activity, produce lipid peroxidation and cause serious membrane injury, suggesting these heavy metals can cause different toxic effects in different ways.