文献类型：期刊文献

英文题名：Establishment of a new cell line from the snout tissue of golden pompano Trachinotus ovatus, and its application in virus susceptibility

作者：Yu, Y.[1,2];Wei, S.[1,2];Wang, Z.[4];Huang, X.[1,2];Huang, Y.[1,2];Cai, J.[5];Li, C.[4];Qin, Q.[1,3]

机构：[1]Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]South China Agr Univ, Coll Marine Sci, Guangzhou 510641, Guangdong, Peoples R China;[4]Qinghai Univ, Coll Ecol Environm Engn, Xining 810016, Peoples R China;[5]Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China

年份：2016

卷号：88

期号：6

起止页码：2251

外文期刊名：JOURNAL OF FISH BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000384301600011)、、WOS

基金：This work was supported by grants from the National High Technology Development Program of China (863) (2014AA093507), the National Basic Research Program of China (973) (2012CB114402), Science and Technology and industrial development Marine fishery Project of Guangdong Province, China (A201501C01), and Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences (LMB141005). The authors would like to thank J. Zhang from Scientific Equipment Service Center of South China Sea Institute of Oceanology for flow cytometry technical assistance.

语种：英文

外文关键词：cell line; snout; transgenic manipulation; virus infection

外文摘要：A new marine-fish cell line, designated GPS, was established from the snout tissue of golden pompano Trachinotus ovatus. GPS cells multiplied well in Leibovitz's L-15 containing 10% foetal bovine serum (FBS) at 28 degrees C and the cells have been subcultured for >60 passages. Polymerase chain reaction (PCR) amplification of 16S ribosomal (r) RNA confirmed the origin of this cell line from T. ovatus. Chromosome analysis showed that GPS cells exhibited chromosomal aneuploidy with a modal chromosome number of 54. Bright green fluorescence signal was observed in enhanced green fluorescent protein (EGFP)-N3 transfected cells, indicating that GPS cells could be used to investigate gene functions in vitro. The GPS cells were highly susceptible to Singapore grouper iridovirus (SGIV), which was demonstrated by the presence of severe cytopathic effect (CPE) and increased viral titres. Real-time quantitative PCR and Western blot analysis showed that the viral gene transcription and protein synthesis occurred during SGIV infection in GPS cells. Thus, this study described the characteristic of a new cell line from the snout tissue of T. ovatus that could be used as a tool for propagation of iridovirus and genetic manipulation to investigate host-pathogen interactions. (C) 2016 The Fisheries Society of the British Isles