文献类型：期刊文献

英文题名：Physicochemical Characterization of Fucoidans from Sargassum henslowianum C.Agardh and Their Antithrombotic Activity In Vitro

作者：Lin, Peichun[1];Chen, Suhua[1];Liao, Min[2];Wang, Weimin[1,2]

机构：[1]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Sch Food Sci & Technol, Zhanjiang 524088, Peoples R China

年份：2022

卷号：20

期号：5

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000801417900001)、、Scopus(收录号：2-s2.0-85129963662)、WOS

基金：The work was supported in part by the Project of Free Application Program of Natural Science Foundation of Guangdong Province, China (2018A0303130020).

语种：英文

外文关键词：Sargassum henslowianum C; Agardh; fucoidan; physicochemical characterization; HUVEC; antithrombotic

外文摘要：Sargassum fucoidan is a kind of sulfated heteropolysaccharide with a variety of biological activities. The aim of this study was to investigate the extraction, purification, physicochemical characterization and in vitro antithrombotic activity of fucoidan from Sargassum henslowianum C.Agardh. Hot-water-assisted ultrasound was used to extract fucoidan (F). Fucoidan was purified by DEAE cellulose 52 (F1), Vc-H2O2 (FD1) and Superdex 75 gel (FDS1). The physical and chemical properties of fucoidans were analyzed by chemical composition, monosaccharide composition, average molecular weight (Mw) and FTIR. The sulfate contents of F, F1, FD1 and FDS1 were 11.45%, 16.35% and 17.52%, 9.66%, respectively; the Mw was 5.677 x 10(5), 4.393 x 10(5), 2.176 x 10(4) and 6.166 x 10(3), respectively. The results of monosaccharide composition showed that the four fucoidans contained l-fucose, d-galactose, l-mannose, d-xylose, l-rhamnose and d-glucose, but the mass fraction ratio was different. The results of FTIR showed that fucoidan contained characteristic peaks of sugar and sulfate. In vitro, F1, FD1 and FDS1 could alleviate HUVEC damage induced by adrenaline (Adr). F1, FD1 and FDS1 decreased vWF and TF and increased the ratio of t-PA/PAI-1 in Adr-induced HUVEC.