文献类型：期刊文献

中文题名：雷州黑鸭FSHR和ESR1与繁殖性状关联分析

英文题名：Correlation Between FSHR and ESR1 and Breeding Traits in Leizhou Black Duck

作者：邹坤[1];崔红艳[1];薛缘[1];张少伟[1];路丽丽[1];赵志辉[1];苏瑛[1]

机构：[1]广东海洋大学农学院

年份：2019

卷号：35

期号：8

起止页码：118

中文期刊名：生物技术通报

外文期刊名：Biotechnology Bulletin

收录：CSTPCD、、北大核心2017、CSCD2019_2020、北大核心、CSCD

基金：广东省科技厅科技计划项目(2017A020208066);广东海洋大学优秀学位论文培育项目(201610)

语种：中文

中文关键词：雷州黑鸭;FSHR;ESR1;SNP;繁殖性状

外文关键词：Leizhou black duck;FSHR;ESR1;SNP;breeding traits

中文摘要：旨为探究产蛋基因FSHR和ESR1对雷州黑鸭的影响。采用荧光定量PCR对FSHR和ESR1在雷州黑鸭0-120d的下丘脑-垂体-卵巢轴的表达规律进行研究,并通过PCR-SSCP对FSHR和ESR1的多态性与繁殖性状关联分析。结果表明,FSHR在0-90d表达量总体呈稳定上升趋势,但在90-120d下丘脑和垂体表达量略微下调,差异不显著(P>0.05);ESR1表达量在0-120d表达量总体呈上升趋势,但在90d卵巢表达量出现波动,差异不显著(P>0.05);FSHR的SNP(g.856937G>A,g.922947A>G)和ESR1的SNP(g.190744A>G)与雷州黑鸭产蛋量显著相关(P<0.05),但与雷州黑鸭蛋品质关联不显著。以上结果提示:FSHR和ESR1对雷州黑鸭早期性腺发育起着重要作用,且3个SNP位点均可作为雷州黑鸭重要的产蛋标记基因。

外文摘要：The aim of this study is to explore effect of the laying-related gene FSHR and ESR1 on Leizhou black duck. Quantitative PCR was employed to investigate the expression pattern of FSHR and ESR1 in hypothalamus-hypophysis-ovary axis of 0-120 d Leizhou black duck,and PCR-SSCP was used for analyzing the correlation between FSHR and ESR1 with breeding traits. The results showed that the overall expression trend of FSHR increased stably at 0-90 d and decreased slightly at 90-120 d in hypothalamus and ovary,but there was no significant difference(P>0.05). The overall expression trend of ESR1 increased in 0-120 d but there was fluctuation at 90 d in ovary and difference was not significant(P>0.05). The SNP for FSHR(g.856937G>A,g.922947A>G)and ESR1(g.190744A>G)were significantly associated with laying performance(P<0.05),but were not correlated with egg quality(P>0.05). The results suggest FSHR and ESR1 play an important role in early gonad development in Leizhou black duck,and three identified SNPs may be used as genotype maker for egg laying.