文献类型：期刊文献

中文题名：转几丁质酶基因水稻抗纹枯病性及农艺性状遗传特性

英文题名：Inheritance analysis on sheath blight resistance and agronomic traits of transgenic rice transformed with chitinsase-gene

作者：袁红旭[1];刘晚苟[1];刘月廉[2];郭建夫[2];李月仁[3]

机构：[1]湛江师范学院生命科学与技术学院,广东湛江524048;[2]广东海洋大学农业生物技术研究所,广东湛江524088;[3]福建省农业科学院中心实验室,福州510275

年份：2012

卷号：43

期号：2

起止页码：151

中文期刊名：南方农业学报

外文期刊名：Journal of Southern Agriculture

收录：CSTPCD、、北大核心2011、CSCD_E2011_2012、北大核心、CSCD

基金：转基因生物工程新品种培育科技重大专项项目(2009ZX08011-02913);湛江师范学院教授专项基金项目[(ZL0806)2009-1]

语种：中文

中文关键词：转几丁质酶基因水稻;海转A;纹枯病抗性;农艺性状;遗传特性

外文关键词：transgenic rice transformed with chitinsase-gene; Haizhuan A; resistance to sheath blight; agronomic trait; inheritance characteristics

中文摘要：【目的】揭示转基因水稻的纹枯病抗性及其非目标变异性状的遗传特性,为合理利用转基因水稻进行抗病育种提供理论指导。【方法】以海转A为父本、竹籼B为母本杂交,通过对杂交F1和F2代的抗病性状进行分析,揭示海转A的纹枯病抗性和部分非目标变异性状的遗传特性。【结果】转基因海转A除抗纹枯病性较非转基因亲本竹籼B有显著提高外,还存在穗粒数和千粒重两个非目标性状的变异,穗粒数较竹籼B增加44.2%,而千粒重降低12.1%。海转A的抗纹枯病性表现为存在主效基因的多基因数量遗传特性,而穗粒数和千粒重表现为典型的数量性状遗传;此外转基因海转A的抗病性、穗粒数和千粒重3个性状的变异系数均显著大于竹籼B,说明其稳定性较低。【结论】外源抗病基因的导入提高了水稻的抗病性,这种影响以主效基因的形式表达;而外源基因导入引起非目标性状的变异,但没有改变其原有的典型数量性状遗传特征。

外文摘要：[Objective]The present experiment was conducted to study the inheritance of sheath blight resistance in transgenic rice and its crossing combinations and variations in some agronomic traits to reasonably apply transgenic rice in breeding of disease resistance.[Method]Transgenic rice line Haizhuan A,transformed with chitinase-gene RC24,was used as male parent to cross with non-transgenic female parent Zhuxian B,and the sheath blight resistance was analyzed in their hybrid F1 and F2 offsprings to find out the inheritance characteristics of sheath blight resistance and variations in agronomic traits in Haizhuan A.[Result]The results showed that,compared to female parent Zhuxian B,transgenic Huaizhuan A showed higher resistance to sheath blight and variations in agronomic traits in terms of 44.2% increase in grain number per panicle and 12.1% decrease in 1000-grain weight.The resistance to sheath blight in Huaizhuan A was due to polygenic quantitative heredity traits located on major genes,while its grain number per panicle and 1000-grain weight still belonged to typical quantitative heredity.Further,the coefficient of variation of resistance and two traits in Haizhuan A were higher than that of Zhuxian B,indicating that their stability decreased remarkably.[Conclusion]It has been observed that some other resistance genes in transgenic rice enhanced its resistance,which was further influenced by transformed gene.Extra resistance gene may induce some variations in agronomic traits,but can not change the inheritance pattern of the traits.