文献类型：期刊文献

英文题名：A mixture of Chinese herbs and a commercial probiotic Bacillus species improves hemato-immunological, stress, and antioxidant parameters, and expression of HSP70 and HIF-1α mRNA to hypoxia, cold, and heat stress in Nile tilapia, Oreochromis niloticus

作者：Abarike, Emmanuel Delwin[1,2,3,4];Jian, Jichang[1,2,3];Tang, Jufen[1,2,3];Cai, Jia[1,2,3];Sakyi, Essien M.[1,2,3];Kuebutornye, Felix K. A.[1,2,3]

机构：[1]Guangdong Ocean Univ, Dept Educ Guangdong Prov, Coll Fishery, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Dept Educ Guangdong Prov, Zhanjiang 524088, Peoples R China;[3]Guangdong Key Lab Control Dis Aquat Econ Anim, Dept Educ Guangdong Prov, Zhanjiang 524088, Peoples R China;[4]Univ Dev Studies, Dept Fisheries & Aquat Resources Management, Tamale, Ghana

年份：2020

卷号：18

外文期刊名：AQUACULTURE REPORTS

收录：SCI-EXPANDED(收录号：WOS:000603386600007)、、WOS

基金：Our study was supported by the National Natural Science Foundation of China (Grant number, 31572651), Natural Science Foundation of Guangdong Province (Grant numbers 2015A030308020 and 2016A030313748) and Science and Technology Planning Project of Guangdong Province (Grant number, 2015A020209181)

语种：英文

外文关键词：Hematology; Antioxidants; Gene expression; Stress; Hypoxia; Cold; Heat

外文摘要：A 56-day feeding trial was performed to evaluate the effect of herbal-probiotic supplements comprising a mixture of Chinese herbs (CH) (i.e., Astragalus membranaceus, Angelica sinensis, and Crataegus hupehensis) and or commercially prepared probiotic Bacillus species (BS) (i.e., spores of B. subtilis and B.licheniformis) on the physiological response of Nile tilapia. Fish (50 +/- 2 g) were fed six diets in duplicates including a control (CT) enriched with BS [at 10 g kg(-1)], CH [at 10 g kg(-1)], CHBS1 [CH at 3 g kg(-1) and BS at 7 g kg(-1)], CHBS2 [CH at 5 g kg(-1) and BS at 5 g kg(-1)], and CHBS3 [CH at 7 g kg(-1) and BS at 3 g kg(-1)] for a period of 56 weeks. At the end of the feeding trial, some hemato-immunological parameters and antioxidant indices, as well as stress-related genes in tilapia, were evaluated; and afterwards exposed to hypoxia for 18 h and to cold and heat each for 2 h. The results showed increased hematocrit, red blood cells, haemoglobin, and white blood cells, in fish blood in most of the herbal-probiotic groups compared to the control group (P < 0.05). Lowered cortisol, glucose, as well pyruvate kinase, alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase, and increased myeloperoxidase, total antioxidant capacity, superoxide dismutase, and catalase activities were observed in the serum of all herbal-probiotic groups over the control group (P < 0.05). Besides, all herbal-probiotic groups showed increased expression of heat shock protein 70 and hypoxia-inducible factor 1 alpha mRNAs in the livers of fish (P < 0.05). Among the herbal-probiotic groups, tilapia fed with CHBS2 diet showed enhanced values in most of the indices measured under the different stress conditions (P < 0.05). These findings suggest CHBS2 supplement as a prophylactic against the suppressive effects of stress in tilapia.