文献类型：期刊文献

英文题名：Immune response and protective efficacy of Streptococcus agalactiae vaccine coated with chitosan oligosaccharide for different immunization strategy in nile tilapia (Oreochromis niloticus)

作者：Lu, Chun-lan[1];Wangkahart, Eakapol[2];Huang, Jun-wei[1];Huang, Yong-xiong[1];Huang, Yu[1];Cai, Jia[1];Jian, Ji-chang[1];Wang, Bei[1]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Anim,Guangdong Prov Key, Zhanjiang, Peoples R China;[2]Mahasarakham Univ, Fac Technol, Div Fisheries, Appl Anim & Aquat Sci Res Unit,Lab Fish Immunol &, Kantarawichai 44150, Mahasarakham, Thailand

年份：2024

卷号：145

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:001156257000001)、、WOS

基金：B.W. supported by the National Natural Science Foundation of China (Grant No. 32002426, U20A2065, 32073006) . Ministry of Science and Technology "Belt and Road" innovation talent exchange foreign experts project (DL202303009) . E.W. was supported by the Office of the Permanent Secretary, Ministry of Higher Education, Science, Research and Innovation, and National Research Council of Thailand (Grant No. RGNS 63-149) . We would like to thank the Analysis and Testing Center of Guangdong Ocean University for providing us with the support of instruments and equipment.

语种：英文

外文关键词：Nile tilapia; S . agalactiae; Chitosan oligosaccharide; Inactivated vaccine; Immunization strategy

外文摘要：In the past decade, the outbreak of Streptococcus agalactiae has caused significant economic losses in tilapia farming. Vaccine immunization methods and strategies have gradually evolved from single-mode to multi-mode overall prevention and control strategies. In this study, an inactivated vaccine of S. agalactiae with a chitosan oligosaccharide (COS) adjuvant was constructed using different administration methods: intraperitoneal injection (Ip), immersion combined with intraperitoneal injection (Im + Ip), immersion combined with oral administration (Im + Or), and oral administration (Or). Safety analysis revealed no adverse effects on tilapia, and the vaccine significantly promoted fish growth and development when administered through Im + Or or Or immunization. Following vaccination, innate immunity parameters including SOD, ACP and CAT activities were all significantly enhanced. Additionally, specific serum IgM antibodies reached their highest level at the 6th week post vaccination. Skin and intestinal mucus IgT antibodies reached peaked at the 6th and 7th week post vaccination, respectively. The relative peak expression values for IL-8, IL-12, MHC-I, MHC-II, IgM, IgT, CD4, CD8, TNF alpha, IFN gamma from Im + Ip group were significantly higher than those in Ip group, Im + Or group and Or group in most cases (p < 0.05). Importantly, the relative protection survival of Im + Ip group was the highest (78.6%), followed by the Ip group (71.4%), the Or group (64.3%) and the Im + Or group (57.1%). In summary, this study encourages further research on multi-channel immunization strategies of other kinds of vaccines in other aquatic economic animals to improve their disease resistance.