文献类型：期刊文献

英文题名：Preventive Effect of YGDEY from Tilapia Fish Skin Gelatin Hydrolysates against Alcohol-Induced Damage in HepG2 Cells through ROS-Mediated Signaling Pathways

作者：Chen, Mei-Fang[1];Gong, Fang[1];Zhang, Yuan Yuan[1];Li, Chengyong[2,3];Zhou, Chunxia[1];Hong, Pengzhi[1];Sun, Shengli[2];Qian, Zhong-Ji[2,3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518114, Peoples R China

年份：2019

卷号：11

期号：2

外文期刊名：NUTRIENTS

收录：SCI-EXPANDED(收录号：WOS:000460829700183)、、Scopus(收录号：2-s2.0-85061855452)、WOS

基金：The study was supported by the Yangfan Scarce Top Talent Project of Guangdong Province (201433009), Program for Scientific Research Start-Up Funds of Guangdong Ocean University [to Zhong-Ji Qian], Innovation and Strong University Project (2013050204) and the Development Project about Marine Economy Demonstration of Zhanjiang City (2017C8B1).

语种：英文

外文关键词：YGDEY; Alcohol metabolism; Oxidative stress; HepG2 cells; DNA damage

外文摘要：According to a previous study, YGDEY from tilapia fish skin gelatin hydrolysates has strong free radical scavenging activity. In the present study, the protective effect of YGDEY against oxidative stress induced by ethanol in HepG2 cells was investigated. First, cells were incubated with YGDEY (10, 20, 50, and 100 M) to assess cytotoxicity, and there was no significant change in cell viability. Next, it was established that YGDEY decreased the production of reactive oxygen species (ROS). Western blot results indicated that YGDEY increased the levels of superoxide dismutase (SOD) and glutathione (GSH) and decreased the expression of gamma-glutamyltransferase (GGT) in HepG2 cells. It was then revealed that YGDEY markedly reduced the expressions of bax and cleaved-caspase-3 (c-caspase-3); inhibited phosphorylation of Akt, IB-, p65, and p38; and increased the level of bcl-2. Moreover, the comet assay showed that YGDEY effectively decreased the amount of ethanol-induced DNA damage. Thus, YGDEY protected HepG2 cells from alcohol-induced injury by inhibiting oxidative stress, and this may be associated with the Akt/nuclear factor-B (NF-B)/mitogen-activated protein kinase (MAPK) signal transduction pathways. These results demonstrate that YGDEY from tilapia fish skin gelatin hydrolysates protects HepG2 cells from oxidative stress, making it a potential functional food ingredient.