文献类型：期刊文献

英文题名：miR-544 Regulates Dairy Goat Male Germline Stem Cell Self-Renewal via Targeting PLZF

作者：Song, Wencong[1];Mu, Hailong[1];Wu, Jiang[2];Liao, Mingzhi[3];Zhu, Haijing[4];Zheng, Liming[1];He, Xin[1];Niu, Bowen[1];Zhai, Yuanxin[1];Bai, Chunling[5];Lei, Anmin[1];Li, Guangpeng[5];Hua, Jinlian[1]

机构：[1]Northwest A&F Univ, Agr Minist China, Key Lab Anim Biotechnol, Shaanxi Ctr Stem Cells Engn & Technol,Coll Vet Me, Yangling 712100, Shaanxi, Peoples R China;[2]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Peoples R China;[3]Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China;[4]Yulin Univ, Coll Life Sci, Yulin Coll, Yulin 719000, Peoples R China;[5]Inner Mongolia Univ, Key Lab Mammalian Reprod Biol & Biotechnol, Minist Educ, Hohhot 010021, Peoples R China

年份：2015

卷号：116

期号：10

起止页码：2155

外文期刊名：JOURNAL OF CELLULAR BIOCHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000359809400005)、、Scopus(收录号：2-s2.0-84939175115)、WOS

基金：Grant sponsor: Program of National Natural Science Foundation of China; Grant number: 31272518; Grant sponsor: National Major Project for Production of Transgenic Breeding; Grant number: 2014ZX08007-002; Grant sponsor: National High Technology Research and Development Program of China; Grant number: SS2014AA021605; Grant sponsor: Doctoral Fund of Ministry of Education of China, RFDP; Grant number: 20120204110030; Grant sponsor: Open Subjects for the Major Basic Researches of Science and Technology Office of Inner Mongolia; Grant number: 20130902.

语种：英文

外文关键词：PLZF; miR-544; MALE GERMLINE STEM CELLS (mGSCs); DAIRY GOAT

外文摘要：The balance between the self-renewal and differentiation of male germline stem cells (mGSCs) is critical for the initiation and maintenance of mammalian spermatogenesis. The promyelocytic leukemia zinc finger (PLZF), a zinc finger protein, is a critical factor for maintaining the self-renewal of mGSCs, so, evaluation of the PLZF pathway in mGSCs may provide a deeper insight into mammalian spermatogenesis. miRNA was also an important regulating factor for the self-renewal and differentiation of mGSCs; however, there is currently no data indicating that which miRNA regulate the self-renewal and differentiation of mGSCs via PLZF. Here, we predicted the prospective miRNA targeting to PLZF using the online Bioinformatics database-Targetscan, and performed an analysis of the dual-luciferase recombinant vector, psiCHCEKTM-2-PLZF-3UTR. miR-544 mimics (miR-544m), miR-544 inhibitors (miR-544i), Control (NC, scrambled oligonucleotides transfection), pPLZF-IRES2-EGFP or PLZF siRNA were transfected into mGSCs; the cells proliferation was evaluated by BRDU incorporation assay and flow cytometry, and the mGSC marker, GFRa1, PLZF, KIT, DAZL, and VASA expression were analyzed by RT-qPCR, immunofluorescence and Western blot. The results showed that miR-544 regulates dairy goat male germline stem cell self-renewal via targeting PLZF. Our study identifies a new regulatory pathway for PLZF and expands upon the PLZF regulatory network in mGSCs. J. Cell. Biochem. 116: 2155-2165, 2015. (c) 2015 Wiley Periodicals, Inc.