文献类型：期刊文献

英文题名：Astaxanthin attenuates ferroptosis via Keap1-Nrf2/HO-1 signaling pathways in LPS-induced acute lung injury

作者：Luo, Lianxiang[1,2];Huang, Fangfang[3];Zhong, Saiyi[4];Ding, Rui[1];Su, Jiating[5];Li, Xiaoling[6]

机构：[1]Guangdong Med Univ, Marine Biomed Res Inst, Zhanjiang 524023, Guangdong, Peoples R China;[2]Marine Biomed Res Inst Guangdong Zhanjiang, Zhanjiang 524023, Guangdong, Peoples R China;[3]Guangdong Med Univ, Grad Sch, Zhanjiang 524023, Guangdong, Peoples R China;[4]Guangdong Ocean Univ, Guangdong Prov Engn Technol Res Ctr Seafood, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Guangdong Prov Engn Lab Marine Biol Prod,Coll Food, Zhanjiang 524088, Peoples R China;[5]Guangdong Med Univ, Clin Coll 1, Zhanjiang 524023, Guangdong, Peoples R China;[6]Guangdong Med Univ, Expt Anim Ctr, Zhanjiang 524023, Guangdong, Peoples R China

年份：2022

卷号：311

外文期刊名：LIFE SCIENCES

收录：SCI-EXPANDED(收录号：WOS:000891202900007)、、Scopus(收录号：2-s2.0-85140960420)、WOS

基金：This project was supported by the Innovative Team Program of High Education of Guangdong Province (2021KCXTD021); The Science and Technology Program of Guangdong Province (2019B090905011); Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety (GDPLLAPPS2104).

语种：英文

外文关键词：Acute lung injury; Astaxanthin; Ferroptosis; Keap1-Nrf2; HO-1; Inflammation

外文摘要：Ferroptosis is a form of regulated nonapoptotic cell death associated with iron-dependent lipid peroxidation. Previous studies have shown that ferroptosis is involved in the occurrence and development of acute lung injury (ALI). In this study, a systems pharmacology approach was performed through the overall process of target acquisition, network construction, and further analysis. Then, the effects of astaxanthin on LPS-induced inflammation and ferroptosis were investigated in RAW264.7 cells induced by LPS in vitro and ALI mice induced by LPS in vivo. The enrichment analysis of astaxanthin-target gene is closely related to the occurrence and development mechanism of ferroptosis. GO and KEGG enrichment analysis of astaxanthin acting on ALI found that these intersection genes are associated with ALI inflammatory pathway. In addition, astaxanthin can effectively inhibit LPS-induced production of pro-inflammatory cytokines and ferroptosis in RAW264.7 cells. Consistently, administration of astaxanthin protected mice against LPS-induced ALI and significantly decreased the extent of lung edema, inflammatory cells infiltration, and ferroptosis in vivo, and Keap1-Nrf2/HO-1 pathway is involved in astaxanthin inhibits LPS-induced ALI and ferroptosis. Taken together, these results demonstrate that astaxanthin inhibit inflammation and ferroptosis by regulating Keap1-Nrf2/HO-1 pathway to reduce LPSinduced ALI.