文献类型：学位论文

中文题名：湛江红树林滩涂真菌群落的分离鉴定和资源调查研究

英文题名：Study on Identification and Resource Investigation Research of Mangrove Fungus from Zhanjiang

作者：殷明亮[1];

机构：[1]广东海洋大学;

导师：于莉;广东海洋大学

授予学位：硕士

语种：中文

中文关键词：红树林;海洋真菌;鉴定;ITS

外文关键词：mangroves;marine fungi;identification;ITS

中文摘要：本文对湛江附近海域红树林,特别是高桥红树林国家自然保护区进行定区域采样,以形态学观察与分子鉴定相结合的方法,初步调查红树林真菌的种类和分布。 三年研究期间,在具亚热带海洋性气候的高桥红树林国家自然保护区,根据红树林保护区内树木种类/(白骨壤、桐花树、秋茄、木榄、漂浮木等/)和潮位带/(低潮位、中潮位、高潮位/)进行分类采集漂流木、固着木与腐叶等采集样品数共计359份,通过分离培养、人工诱培、形态学观察以及ITS分子测序鉴定技术,获得菌株数共计78株,鉴定完毕45株,分别隶属于18个属38个种真菌,待鉴定7株,鉴定的种属有:Guignardia ardisiae、Glomerella cingulata、Chaetomium globosum、C.murorum、Eutypella scoparia、Pestalotiopsis microspora、Eurotium amstelodami、E. rubrum、Eupenicillium brefeldianum、Phomopsis liquidambari、Myrothecium inundatum、Nigrospora oryzae、Paecilomyces lilacinus、Alternaria arborescens、Cladosporium cladosporioides、Aspergillus niger、A. versicolor、A. Caesiellus、Penicillium griseofulvum、P. expansum、P. implicatum、P. janthinellum、P. herquei、P. glabrum、P. verruculosum、Fusarium equiseti、F. oxysporum、Trichoderma harzianum、T. atroviride、T. saturnisporum、T. spirale、T. Asperellum、Pichia guilliermondii. 以白骨壤为代表树种的低潮位带优势属为青霉属/(Penicillium/),其次为木霉属/(Trichoderma/)和镰孢属/(Fusarium/);以秋茄为代表树种的中潮位带优势属为青霉属/(Penicillium/),其次为毛壳属/(Chaetomium/)和拟茎点霉属/(Phomopsis/);以木榄为代表树种的高潮位优势属为青霉属/(Penicillium/)和木霉属/(Trichoderma/)。

外文摘要：In this paper,we collect samples from Gaoqiao Mangrove National Nature Reserve area,identify fungus by morphological and molecular methods,in order to the preliminary investigation of species and distribution of mangrove fungus. During past three years, 359 samples have been collected from Gaoqiao Mangrove National Nature Reserve, including drift wood solid wood and fallen rotten leaves,etc according to the types of trees in protected areas of mangrove and the tidal zone for classified collection. Based on morphological characteristics and sequence analysis of rDNA ITS region, 78 typic strains, 39 species of 18 genera were identified:Guignardia ardisiae、Glomerella cingulata、Chaetomium globosum、C.murorum、Eutypella scoparia、Pestalotiopsis microspora、Eurotium amstelodami、E. rubrum、Eupenicillium brefeldianum、Phomopsis liquidambari、Myrothecium inundatum、Nigrospora oryzae、Paecilomyces lilacinus、Alternaria arborescens、Cladosporium cladosporioides、Aspergillus niger、A. versicolor、A. Caesiellus、Penicillium griseofulvum、P. expansum、P. implicatum、P. janthinellum、P. herquei、P. glabrum、P. verruculosum、Fusarium equiseti、F. oxysporum、Trichoderma harzianum、T. atroviride、T. saturnisporum、T. spirale、T. Asperellum、Pichia guilliermondii. The dominant genera of low tide zone is Penicillium, followed by Trichoderma and Fusarium genus;The dominant genera of mid tide zone is Penicillium, followed by Chaetomium and Phomopsis genus; The dominant genera of high tide zone is Penicillium, followed by Trichoderma genus;The dominant genera of Avicennia marina habitat is Penicillium, followed by Fusarium genus; The dominant genera of Aegiceras corniculatum habitat is Phomopsis, followed by Chaetomium genus;The dominant genera of Kandelia obovata habitat is Penicillium, followed by Chaetomium genus;The dominant genera of Bruguiera gymnorrhiza habitat is Trichoderma.

年份：2010