文献类型：期刊文献

英文题名：Identification, characterization and functional analysis of a serine protease inhibitor (Lvserpin) from the Pacific white shrimp, Litopenaeus vannamei

作者：Liu, Yongjie[1];Hou, Fujun[1];He, Shulin[1];Qian, Zhaoying[1];Wang, Xianzong[1];Mao, Aitao[2];Sun, Chengbo[2];Liu, Xiaolin[1]

机构：[1]Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524025, Guangdong, Peoples R China

年份：2014

卷号：43

期号：1

起止页码：35

外文期刊名：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000331479800006)、、WOS

基金：We thank the professor Fuhua Li for kindly giving the three pathogens. This work was supported by the Agricultural Science and Technology Achievement Transformation Fund Project of Ministry of Science and Technology, China (No. 2012GB2E200361), the National Fundamental Research Program, China (No. 2006AA10A406), the Key laboratory of Marine Biology, Institute of Oceanology, Chinese Academy of Sciences and the Northwest A&F University experimental demonstration station (base) and innovation of science and technology achievement transformation project (No. XNY2013-4).

语种：英文

外文关键词：Litopenaeus vannamei; Serpin; RNA interference; Prophenoloxidase; Immunity

外文摘要：As important arthropod immune responses, prophenoloxidase (proPO) activation and Toll pathway initiation are mediated by serine proteinase cascades and regulated by serpins. Herein, a serine protease inhibitor (Lvserpin), encoding for 415 amino acids with calculated molecular weight of 46,639 Da and iso-electric point of 7.03 was characterized from the Pacific white shrimp Litopenaeus vannamei. Multiple sequence alignment revealed that Lvserpin shared the highest similarity with Penaeus monodon serpin6 (87%). Quantitative real-time PCR (qRT-PCR) results showed that the transcripts of Lvserpin were detected in all the examined tissues and most highly expressed in gill. The expression profiles of Lvserpin were greatly fluctuated upon infection of Vibrio anguillarum, Micrococcus lysoleikticus or White Spot Syndrome Virus (WSSV). Double stranded RNA-mediated suppression of Lvserpin resulted in a significant increase in the transcripts of two clip-domain serine proteinases (PPAE and PPAF), prophenoloxidase (proPO), antilipopolysaccharide factor CALF), Crustin and penaeidin3 (Pens3) and also increased the high cumulative mortality post V. anguillarum injection. Besides, the recombinant Lvserpin protein (rLvserpin) was purified and exhibited inhibitory activity against trypsin. Also the rLvserpin showed inhibition on prophenoloxidase activation and bacterial growth. Hence, we proposed that the Lvserpin played important role in the shrimp innate immunity. (C) 2013 Elsevier Ltd. All rights reserved.