文献类型：期刊文献

中文题名：抗溶藻弧菌的芽孢杆菌的筛选、鉴定及其生物学特性分析

英文题名：Screening and identification of Bacillus strain against Vibrio alginolyticus and biological characteristics analysis

作者：王琳晶[1];丁燏[1]

机构：[1]广东海洋大学水产学院广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524000

年份：2022

卷号：45

期号：10

起止页码：61

中文期刊名：饲料研究

外文期刊名：Feed Research

收录：CSTPCD、、北大核心、北大核心2020

基金：广东省自然科学基金项目(项目编号:2014A030313604);广东海洋大学研究生教育创新计划资助项目(项目编号:201724)。

语种：中文

中文关键词：拮抗作用;溶藻弧菌;解淀粉芽孢杆菌;生物学特性

外文关键词：antagonistic effect;Vibrio alginolyticus;Bacillus amyloliquefaciens;biological characteristics

中文摘要：试验筛选对溶藻弧菌HY9901具有拮抗作用的芽孢杆菌。于广东湛江海域的红树林根部土壤中分离出117株芽孢杆菌,通过初筛和复筛,获得1株对溶藻弧菌HY9901具有较强抑制作用的ZJ2009菌株,对其鉴定以及生物学特性分析。结果显示:ZJ2009菌株为解淀粉芽孢杆菌;在瓶装载量10%~20%,初始pH值6.0~8.0、添加1.5%NaCl的BHI培养基中培养12 h以上生长最佳;该菌株具有广谱抑菌性,无溶血现象,对斑马鱼无毒副作用且具有一定的保护率。研究表明,解淀粉芽孢杆菌用于抑制溶藻弧菌,为弧菌病的防治提供新思路,在水产养殖领域具有开发潜能。

外文摘要：The study was to screen Bacillus antagonistic to Vibrio alginolyticus HY9901. A total of 117 strains of Bacillus were isolated from the root soil of mangrove in Zhanjiang, Guangdong province. A ZJ2009 strain with strong inhibitory effect on V. alginolyticus was obtained through primary and secondary screening. Its identification and biological characteristics were analyzed. The results showed that ZJ2009 strain was identified as Bacillus amyloliquefaciens. The optimal growth condition was BHI medium with 10% to 20% bottled load, initial pH value 6.0 to 8.0 and 1.5% NaCl added for more than 12 h. The strain had broad spectrum bacteriostasis, and failed to produce hemolytic loops, with less side effects on zebrafish and has a certain protection rate. The experiment indicates that Bacillus amyloliquefaciens can inhibit V. alginolyticus and provide a new idea for the prevention and control of vibriosis, which has potential development in the field of aquaculture.